Version 1
: Received: 6 November 2022 / Approved: 7 November 2022 / Online: 7 November 2022 (04:31:29 CET)
How to cite:
Wang, B. Chloroplast Genetics Engineering: from Bench to Blast Application of Chloroplast Genetic-Engineering in Basic Research. Preprints2022, 2022110106. https://doi.org/10.20944/preprints202211.0106.v1
Wang, B. Chloroplast Genetics Engineering: from Bench to Blast Application of Chloroplast Genetic-Engineering in Basic Research. Preprints 2022, 2022110106. https://doi.org/10.20944/preprints202211.0106.v1
Wang, B. Chloroplast Genetics Engineering: from Bench to Blast Application of Chloroplast Genetic-Engineering in Basic Research. Preprints2022, 2022110106. https://doi.org/10.20944/preprints202211.0106.v1
APA Style
Wang, B. (2022). Chloroplast Genetics Engineering: from Bench to Blast Application of Chloroplast Genetic-Engineering in Basic Research. Preprints. https://doi.org/10.20944/preprints202211.0106.v1
Chicago/Turabian Style
Wang, B. 2022 "Chloroplast Genetics Engineering: from Bench to Blast Application of Chloroplast Genetic-Engineering in Basic Research" Preprints. https://doi.org/10.20944/preprints202211.0106.v1
Abstract
Abstract: Chloroplast is a new hotspot in the field of plant transformation system of plant genetic engineering. Initially developed in Chlamydomonas and tobacco, it is now feasible in a broad range of species. They exploit the homologous recombination and segregation pathways acting on chloroplast genomes and are based on direct repeats, transient co-integration or co-transformation and segregation of trait and marker genes. Foreign site-specific recombinases and their target sites provide an alternative and effective method for removing marker genes from plastids.Chloroplast genetic engineering has many advantages over nuclear genetic enginering, especially site-specific introduction of foreign genes ,leading to the absence of gene siliency and positon effect,which providing the available to explore the regulation and mechanism of chloroplast genes`expression in vitro .It also can identify the structure and function of the chloroplast genome , the expression of chloroplast affect the nulear genome. In this paper, the basic methodology of chloroplast transformation, the current techniques and applications, and the future possibilities for Chloroplast genetic engineering was reviewed[1-3].
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
