Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Early and Sustained Inflammatory State Induces Muscle Changes and Establishes Obesogenic Characteristics in Wistar Rats Exposed to MSG-Induced Obesity Model

Matheus Felipe Zazula
* ORCID logo ,
Diego Francis Saraiva
,
João Lucas Theodoro
ORCID logo ,
Mônica Maciel
,
Eliel Vieira dos Santos Sepulveda
,
Bárbara Zanardini de Andrade
ORCID logo ,
Mariana Laís Boaretto
,
Jhyslayne Ignácia Hoff Nunes Maciel
,
Gabriela Alves Bronczek
,
Gabriela Moreira Soares
,
Sara Cristina Sagae Schneider
,
Gladson Ricardo Flor Bertolini
ORCID logo ,
Márcia Miranda Torrejais
,
Lucineia Fatima Chasko Ribeiro
,
Luiz Claudio Fernandes
,
Katya Naliwaiko
Version 1 : Received: 21 October 2022 / Approved: 31 October 2022 / Online: 31 October 2022 (01:00:42 CET)

A peer-reviewed article of this Preprint also exists.

Zazula, M.F.; Saraiva, D.F.; Theodoro, J.L.; Maciel, M.; Sepulveda, E.V.S.; Zanardini de Andrade, B.; Boaretto, M.L.; Maciel, J.I.H.N.; Bronczek, G.A.; Soares, G.M.; Sagae Schneider, S.C.; Bertolini, G.R.F.; Torrejais, M.M.; Ribeiro, L.F.C.; Fernandes, L.C.; Naliwaiko, K. An Early and Sustained Inflammatory State Induces Muscle Changes and Establishes Obesogenic Characteristics in Wistar Rats Exposed to the MSG-Induced Obesity Model. Int. J. Mol. Sci. 2023, 24, 4730. Zazula, M.F.; Saraiva, D.F.; Theodoro, J.L.; Maciel, M.; Sepulveda, E.V.S.; Zanardini de Andrade, B.; Boaretto, M.L.; Maciel, J.I.H.N.; Bronczek, G.A.; Soares, G.M.; Sagae Schneider, S.C.; Bertolini, G.R.F.; Torrejais, M.M.; Ribeiro, L.F.C.; Fernandes, L.C.; Naliwaiko, K. An Early and Sustained Inflammatory State Induces Muscle Changes and Establishes Obesogenic Characteristics in Wistar Rats Exposed to the MSG-Induced Obesity Model. Int. J. Mol. Sci. 2023, 24, 4730.

Abstract

The model of obesity induced by monosodium glutamate cytotoxicity on the hypothalamic nuclei is widely used in the literature. However, MSG promotes persistent muscle changes and there is a significant lack of studies that seek to elucidate the mechanisms by which damage refractory to reversal is established. This study aimed to investigate the early and chronic effects of MSG in-duction of obesity upon systemic and muscular parameters of Wistar rats. Animals were exposed to MSG subcutaneously (4 mg.g-1 b.w.) or saline (1.25 mg.g-1 b.w.) daily from PND01 to PND05 (n = 24). After, in PND15, 12 animals were euthanized to determine the plasma and inflammatory profile and to assess muscle damage. In PND142, the remaining animals were euthanized, and samples for histological and biochemical analyses were obtained. Our results suggest that early exposure to MSG reduced growth, and increased adiposity, induction hyperinsulinemia, and a pro-inflammatory scenario. In adulthood were found, peripheral insulin resistance, reduced muscle mass, oxidative capacity, neuromuscular junctions, increased fibrosis, and oxidative distress. Thus, we can conclude that the condition found in adult life and the difficulty in restoring the muscle profile are related to the metabolic damages established early.

Keywords

hyperinsulinemia; monosodium glutamate; metabolic syndrome; distress oxidative; pro-inflammatory profile; skeletal muscle fibre types.

Subject

Biology and Life Sciences, Anatomy and Physiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.