Version 1
: Received: 25 October 2022 / Approved: 26 October 2022 / Online: 26 October 2022 (10:15:55 CEST)
How to cite:
Kabtani, J.; BOULANOUAR, F.; Militiello, M.; Cassagne, C.; Ranque, S. CandidaMassiliensis sp. nov. Isolated from A Clinical Sample. Preprints2022, 2022100412. https://doi.org/10.20944/preprints202210.0412.v1
Kabtani, J.; BOULANOUAR, F.; Militiello, M.; Cassagne, C.; Ranque, S. Candida Massiliensis sp. nov. Isolated from A Clinical Sample. Preprints 2022, 2022100412. https://doi.org/10.20944/preprints202210.0412.v1
Kabtani, J.; BOULANOUAR, F.; Militiello, M.; Cassagne, C.; Ranque, S. CandidaMassiliensis sp. nov. Isolated from A Clinical Sample. Preprints2022, 2022100412. https://doi.org/10.20944/preprints202210.0412.v1
APA Style
Kabtani, J., BOULANOUAR, F., Militiello, M., Cassagne, C., & Ranque, S. (2022). <i>Candida</i> <i>Massiliensis</i> sp. nov. Isolated from A Clinical Sample. Preprints. https://doi.org/10.20944/preprints202210.0412.v1
Chicago/Turabian Style
Kabtani, J., Carole Cassagne and Stéphane Ranque. 2022 "<i>Candida</i> <i>Massiliensis</i> sp. nov. Isolated from A Clinical Sample" Preprints. https://doi.org/10.20944/preprints202210.0412.v1
Abstract
The majority of Candida species are known as non-pathogenic yeasts and rarely involved in human diseases. However, recently case reports of human infections caused by non-albicans Candida species have increased, mostly in immunocompromised hosts. Our study aimed to describe and caracterise as thoroughly as possible, a new species of the Candida genus, named here Candida massiliensis (PMML0037), isolated from a clinical sample of human sputum. We compared genetic data based on the sequences of four genetic regions: "Internal Transcribed Spacers" of rRNA, D1/D2 domains (28S large subunit rRNA) and part of the genes encoding Translation Elongation Factor 1-α and β-tubulin2, to morphological characters, from scanning electron microscopy (TM 4000 Plus, SU5000), physiological, including the results of oxidation and assimilation tests of different carbon sources by the Biolog system, and chemical mapping by Energy-Dispersive X-ray Spectroscopy. Lastly, the in vitro antifungal susceptibility profile was performed using the E-testTM exponential gradient method. The multilocus analysis supported the genetic position of Candida massiliensis (PMML0037) as a new species of the genus Candida, and the phenotypic analysis highlighted its unique morphological and chemical profile when compared to other Candida species included in the study.
Keywords
biolog phenotypic technology; Candida; energy-dispersive X-ray spectroscopy; genotype; multilocus DNA sequencing; one new taxon; yeast
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
