Yoshino, Y.; Miyaji, H.; Nishida, E.; Kanemoto, Y.; Hamamoto, A.; Kato, A.; Sugaya, T.; Akasaka, T. Periodontal Tissue Regeneration by Recombinant Human Collagen Peptide Granules Applied with β-Tricalcium Phosphate Fine Particles. Journal of Oral Biosciences, 2023, 65, 62–71. https://doi.org/10.1016/j.job.2023.01.002.
Yoshino, Y.; Miyaji, H.; Nishida, E.; Kanemoto, Y.; Hamamoto, A.; Kato, A.; Sugaya, T.; Akasaka, T. Periodontal Tissue Regeneration by Recombinant Human Collagen Peptide Granules Applied with β-Tricalcium Phosphate Fine Particles. Journal of Oral Biosciences, 2023, 65, 62–71. https://doi.org/10.1016/j.job.2023.01.002.
Yoshino, Y.; Miyaji, H.; Nishida, E.; Kanemoto, Y.; Hamamoto, A.; Kato, A.; Sugaya, T.; Akasaka, T. Periodontal Tissue Regeneration by Recombinant Human Collagen Peptide Granules Applied with β-Tricalcium Phosphate Fine Particles. Journal of Oral Biosciences, 2023, 65, 62–71. https://doi.org/10.1016/j.job.2023.01.002.
Yoshino, Y.; Miyaji, H.; Nishida, E.; Kanemoto, Y.; Hamamoto, A.; Kato, A.; Sugaya, T.; Akasaka, T. Periodontal Tissue Regeneration by Recombinant Human Collagen Peptide Granules Applied with β-Tricalcium Phosphate Fine Particles. Journal of Oral Biosciences, 2023, 65, 62–71. https://doi.org/10.1016/j.job.2023.01.002.
Abstract
Recombinant human collagen peptide (RCP) is a recombinantly created xeno-free biomaterial enriched in RGD (arginine-glycine-aspartic acid) sequences, with good processability that is being investigated for regenerative medicine applications. Recently, the biocompatibility and osteogenic ability of β-TCP/RCP (RCP granules combined with β-tricalcium phosphate (TCP) submicron particles) were demonstrated. In the present study, β-TCP/RCP was implanted into experimental periodontal tissue defects (three-walled bone defect) created in beagle dogs to investigate tissue responses and subsequent regenerative effects. Micro computed tomography image analysis at 8 weeks postoperatively showed that the amount of new bone after β-TCP/RCP graft was significantly greater (2.2 fold, P<0.05) than that of the control (no graft) group. Histological findings showed that the transplanted β-TCP/RCP induced active bone-like tissue formation including TRAP-positive and OCN-positive cells as well as bioabsorbability. Ankylosis did not occur, and periostin-positive periodontal ligament-like tissue formation was observed. Histological measurements revealed that β-TCP/RCP implantation formed 1.7-fold more bone-like tissue and 2.1-fold more periodontal ligament-like tissue than the control, and significantly suppressed gingival recession and epithelial downgrowth (P<0.05). These results suggest that β-TCP/RCP is effective as a periodontal tissue regenerative material.
Medicine and Pharmacology, Dentistry and Oral Surgery
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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