Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Repeated Intravaginal Inoculation of Zika Virus Protects Cynomolgus Monkeys from Subcutaneous Superchallenge

Maya Shofa
ORCID logo ,
Tomotaka Okamura
,
Emiko Urano
,
Yasuhiro Yasutomi
* ,
Yoshiharu Matsuura
* ,
Akatsuki Saito
* ORCID logo
Version 1 : Received: 17 October 2022 / Approved: 18 October 2022 / Online: 18 October 2022 (07:45:22 CEST)

A peer-reviewed article of this Preprint also exists.

Shofa, M.; Okamura, T.; Urano, E.; Matsuura, Y.; Yasutomi, Y.; Saito, A. Repeated Intravaginal Inoculation of Zika Virus Protects Cynomolgus Monkeys from Subcutaneous Superchallenge. Int. J. Mol. Sci. 2022, 23, 14002. Shofa, M.; Okamura, T.; Urano, E.; Matsuura, Y.; Yasutomi, Y.; Saito, A. Repeated Intravaginal Inoculation of Zika Virus Protects Cynomolgus Monkeys from Subcutaneous Superchallenge. Int. J. Mol. Sci. 2022, 23, 14002.

Abstract

Zika virus (ZIKV) outbreaks in Central and South America caused severe public health problems in 2015 and 2016. These outbreaks were finally contained through several methods, including mosquito control using insecticides and repellents. Additionally, the development of herd immunity in these countries might have contributed to containing the epidemic. While ZIKV is mainly transmitted by mosquito bites, mucosal transmission via bodily fluids, including the semen of infected individuals, has also been reported. We evaluated the effect of mucosal ZIKV infection on continuous subcutaneous challenges in a cynomolgus monkey model. Repeated intravaginal inoculations of ZIKV did not induce detectable viremia or clinical symptoms, and all animals developed a potent neutralizing antibody, protecting animals from the subsequent subcutaneous superchallenge. These results suggest that viral replication at mucosal sites can induce protective immunity without causing systemic viremia or symptoms.

Keywords

Zika virus; intravaginal infection; superchallenge; neutralizing antibody

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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