Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Copaiba Oilresin Exerts an Additive Effect to the Babassu Oil on Behavioral Changes in Human Endometriotic Cell Cultures

Julianna Henriques da Silva
,
Leticia Coli Louvisse de Abreu
,
Renato Ferrari
,
Célia Yelimar Palmero
,
Eliane Gouvêa de Oliveira Barros
ORCID logo ,
Natália de Moraes Cordeiro
,
Bruno Pontes
ORCID logo ,
Valeria Pereira de Sousa
,
Lucio Mendes Cabral
,
Patricia Dias Fernandes
* ORCID logo ,
Luiz Eurico Nasciutti
Version 1 : Received: 11 October 2022 / Approved: 17 October 2022 / Online: 17 October 2022 (02:07:50 CEST)

A peer-reviewed article of this Preprint also exists.

Silva, J.H.; Abreu, L.C.L.; Ferrari, R.; Quintana, C.Y.P.; Barros, E.G.O.; Cordeiro, N.M.; Pontes, B.; Sousa, V.P.; Cabral, L.M.; Fernandes, P.D.; Nasciutti, L.E. Copaiba Oil Resin Exerts an Additive Effect to Babassu Oil on Behavioral Changes in Human Endometriotic Cell Cultures. Pharmaceuticals 2022, 15, 1414. Silva, J.H.; Abreu, L.C.L.; Ferrari, R.; Quintana, C.Y.P.; Barros, E.G.O.; Cordeiro, N.M.; Pontes, B.; Sousa, V.P.; Cabral, L.M.; Fernandes, P.D.; Nasciutti, L.E. Copaiba Oil Resin Exerts an Additive Effect to Babassu Oil on Behavioral Changes in Human Endometriotic Cell Cultures. Pharmaceuticals 2022, 15, 1414.

Abstract

Background: Current drug for the treatment of endometriosis is not able of completely cure and significant side effects hinder the continuation of treatment. So, the search for new drug candidates is necessary, and the use of plants extracts is highlighted. Babassu oil and copaiba oilresin have several therapeutic properties. We investigated the in vitro effects of two nanoemulsions containing babassu oil (Orbignya speciosa) (SNEDDS-18) and/or copaiba oilresin (Copaifera langsdorffii) (SNEDDS-18/COPA) on cultured human eutopic endometrium stromal cells from endometrial biopsies of patients without (CESC) and with (EuESC) endometriosis, and human stromal cells from biopsies of endometriotic lesions (EctESC). Methods: CESC, EuESC and EctESC were established and treated with SNEDDS-18 and SNEDDS-18/COPA to evaluate its effects on cytotoxicity, cell morphology, proliferation, and signaling pathways. Results: After 48 hours incubation with SNEDDS-18 and SNEDDS-18/COPA, cell viability and proliferation were inhibited, especially in EctESC. The lowest concentration of both nanoemulsions reduced cell viability and proliferation and breakdown the cytoskeleton in EctESCs. After 24h treatment it was observed a decrease in IL-1, TNF-α, and MCP-1, and an increase in IL-10 production. Conclusions: Both nanoemulsions can affect the endometriotic stromal cell behaviors, thus revealing two potential candidates for new phytotherapeutic agents on the management of endometriosis.

Keywords

Endometriosis; Orbignya speciosa; Copaifera lansgdorffii nanoemulsion; self-nanoemulsifying drug delivery system

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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