PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Spatial Distribution and Genetic Diversity of The Fall Armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) in the Bali Province and Lesser Sunda, Indonesia
Version 1
: Received: 1 October 2022 / Approved: 4 October 2022 / Online: 4 October 2022 (11:02:51 CEST)
How to cite:
Supartha, I. W.; Susila, I. W.; Yudha, I. K. W.; Utama, I. W. E. K.; Sandikayasa, I. W.; Mahendra Putra Gautama, I. P.; Wahyuni, S.; Angga Wiradana, P. Spatial Distribution and Genetic Diversity of The Fall Armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) in the Bali Province and Lesser Sunda, Indonesia. Preprints2022, 2022100020. https://doi.org/10.20944/preprints202210.0020.v1
Supartha, I. W.; Susila, I. W.; Yudha, I. K. W.; Utama, I. W. E. K.; Sandikayasa, I. W.; Mahendra Putra Gautama, I. P.; Wahyuni, S.; Angga Wiradana, P. Spatial Distribution and Genetic Diversity of The Fall Armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) in the Bali Province and Lesser Sunda, Indonesia. Preprints 2022, 2022100020. https://doi.org/10.20944/preprints202210.0020.v1
Supartha, I. W.; Susila, I. W.; Yudha, I. K. W.; Utama, I. W. E. K.; Sandikayasa, I. W.; Mahendra Putra Gautama, I. P.; Wahyuni, S.; Angga Wiradana, P. Spatial Distribution and Genetic Diversity of The Fall Armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) in the Bali Province and Lesser Sunda, Indonesia. Preprints2022, 2022100020. https://doi.org/10.20944/preprints202210.0020.v1
APA Style
Supartha, I. W., Susila, I. W., Yudha, I. K. W., Utama, I. W. E. K., Sandikayasa, I. W., Mahendra Putra Gautama, I. P., Wahyuni, S., & Angga Wiradana, P. (2022). Spatial Distribution and Genetic Diversity of The Fall Armyworm, <em>Spodoptera frugiperda</em> J.E. Smith (Lepidoptera: Noctuidae) in the Bali Province and Lesser Sunda, Indonesia. Preprints. https://doi.org/10.20944/preprints202210.0020.v1
Chicago/Turabian Style
Supartha, I. W., Sri Wahyuni and Putu Angga Wiradana. 2022 "Spatial Distribution and Genetic Diversity of The Fall Armyworm, <em>Spodoptera frugiperda</em> J.E. Smith (Lepidoptera: Noctuidae) in the Bali Province and Lesser Sunda, Indonesia" Preprints. https://doi.org/10.20944/preprints202210.0020.v1
Abstract
Spodoptera frugiperda is an invasive pest that has spread in various parts of the world. These pests have the ability to spread and adapt highly to new habitats. Until now, it is not known with certainty the distribution of these invasive pests in Eastern Indonesia, especially Bali and Nusa Tenggara. This study aims to map the spatial distribution and genetic distribution of S. frugiperda which damages maize in the areas of Bali and Nusa Tenggara. This research was conducted using a survey method from May to September 2022 covering the islands of Bali, Lombok, Sumbawa, Sumba, Flores, and Timor. The results showed that S. frugiperda had spread evenly in Bali and Nusa Tenggara. The results of PCR amplification in the COI gene from 9 sample isolates from all research locations showed the similarity of DNA bands leading to the Spodoptera frugiperda species with a banding pattern length of 683 – 697. These results indicated that the distribution of genetic variants of corn caterpillars in Bali, NTB, and NTT was confirmed as S. frugiperda species. However, the isolated gene S. frugiferda, which was shown by the alignment results of the sequences from Lombok, was confirmed as a different strain from strains from Bali, Sumba, Sumbawa, Flores, and Timor. This incident can be seen from the difference in the protein base composition of S. frugiperda from Bali, Sumba, Sumbawa, Timor, and Flores. The results of phylogenetic analysis in this study confirmed 3 clusters of the genetic closeness of S. frugiperda. Cluster-1 comes from the results of the search for specimens of JB FAW and KB FAW from Bali, SB FAW and SB FAW Sorghum from Sumba, SW FAW from Sumbawa, KP FAW from Timor, and FL FAW from Flores. Cluster-2 is an isolate outside of our species. Cluster-3 comes from the search for LT and LS FAW specimens from Lombok. The genetic distance between cluster-1 and cluster-3 is quite far, which is 0.20 mu.
Keywords
The Fall Armyworm; Spodoptera frugiperda; invasive species; mapping distribution; DNA-barcoding
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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