Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Investigation of Changes in Spermatozoa Characteristics, Chromatin Structure, and Antioxidant/Oxidant Parameters after Freeze-Thawing of Hesperidin (Vitamin P) Doses Added to Ram Semen

Version 1 : Received: 27 September 2022 / Approved: 28 September 2022 / Online: 28 September 2022 (09:35:03 CEST)

A peer-reviewed article of this Preprint also exists.

Yeni, D.; Güngör, Ş.; Avdatek, F.; Gülhan, M.F.; Olğaç, K.T.; İnanç, M.E.; Denk, B.; Taşdemir, U. Investigation of Changes in Spermatozoon Characteristics, Chromatin Structure, and Antioxidant/Oxidant Parameters after Freeze-Thawing of Hesperidin (Vitamin P) Doses Added to Ram Semen. Life 2022, 12, 1780. Yeni, D.; Güngör, Ş.; Avdatek, F.; Gülhan, M.F.; Olğaç, K.T.; İnanç, M.E.; Denk, B.; Taşdemir, U. Investigation of Changes in Spermatozoon Characteristics, Chromatin Structure, and Antioxidant/Oxidant Parameters after Freeze-Thawing of Hesperidin (Vitamin P) Doses Added to Ram Semen. Life 2022, 12, 1780.

Abstract

We conducted this study to determine the potential cryopreservative effects of different hesperidin (vitamin P; HSP) doses on ram semen after freeze-thawing. Semen samples were obtained from Sönmez rams by an artificial vagina. The samples were divided into six groups: control, 10, 50, 100, 250, and 500 µg/mL HSP (C, HSP10, HSP50, HSP100, HSP250, and HSP500, respectively). At the end of the study, sperm motility and kinetic parameters, plasma membrane acrosome integrity (PMAI), high mitochondrial membrane potential (HMMP), viability, lipid peroxidation levels (LPL), chromatin damage, oxidant parameters, and antioxidant parameters were assayed. None of the doses of HSP added to the semen extender showed any enhancing effect on progressive motility compared to C (p>0.05). In fact, HSP500 had negative effects (p<0.05). Moreover, PMI activities were the highest at the HSP10 dose, while LPL values were the lowest (p<0.05). The doses of HSP10 and HSP50 added to the Tris extender medium showed positive effects on spermatozoon chromatin damage. Consequently, we can say that HSP doses used in this study are not effective on semen progressive motility, but the HSP10 dose is effective on PMAI and chromatin damage by reducing LPL.

Keywords

Semen; hesperidin; cryopreservation; oxidative stress; antioxidant; ram

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

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