Uncultured, unmodified, autologous, adipose-derived regenerative cells (UA-ADRCs) are a safe and effective treatment option for various musculoskeletal pathologies. However, it is unknown whether the composition of the final cell suspension systematically varies with the subject's individual age, gender, body mass index (BMI) and ethnicity. UA-ADRCs were isolated from lipoaspirate from n=232 subjects undergoing elective lipoplasty using the Transpose RT system (InGeneron, Inc.; Houston, TX, USA). The UA-ADRCs were assessed for the number of nucleated cells, cell viability and the number of viable nucleated cells per gram of adipose tissue harvested. Flow cytometry was used to further characterize the UA-ADRCs. UA-ADRCs isolated with the Transpose RT system (InGeneron) can be recommended for all ages, genders, BMIs and ethnicities. Furthermore, it was found that for all experimental methods and commerically available systems described in the literature to isolate UA-ADRCs or systems that provide non-enzymatic, mechanical dissociation of lipoaspirate, the Transpose RT system yielded the highest mean relative numbers of CD45- / CD31- / CD34+ cells (adipose tissue derived progenitors), CD45- / CD31+ / CD34+ cells (endothelial progenitors) and CD45+ / CD206+ cells (M2 macrophages). Application of these cell types may significantly contribute to tissue regeneration.