Preprint Article Version 2 Preserved in Portico This version is not peer-reviewed

Isolation and Characterization of Staphylococcus Phage Rih21 and Evaluation of its Antibacterial Activity against Methicillin-resistant Staphylococcus aureus Clinical Isolates

Version 1 : Received: 30 August 2022 / Approved: 1 September 2022 / Online: 1 September 2022 (04:11:06 CEST)
Version 2 : Received: 1 October 2022 / Approved: 5 October 2022 / Online: 5 October 2022 (10:13:04 CEST)

How to cite: Ghayyib, A. A.; Ahmed, I. A.; Ahmed, H. K. Isolation and Characterization of Staphylococcus Phage Rih21 and Evaluation of its Antibacterial Activity against Methicillin-resistant Staphylococcus aureus Clinical Isolates. Preprints 2022, 2022090004. https://doi.org/10.20944/preprints202209.0004.v2 Ghayyib, A. A.; Ahmed, I. A.; Ahmed, H. K. Isolation and Characterization of Staphylococcus Phage Rih21 and Evaluation of its Antibacterial Activity against Methicillin-resistant Staphylococcus aureus Clinical Isolates. Preprints 2022, 2022090004. https://doi.org/10.20944/preprints202209.0004.v2

Abstract

From the hospital wastewater, a novel bacteriophage was isolated and characterized. According to characterization properties, this bacteriophage belongs to the Siphoviridae family, the maximum bacteriophage titer was recorded at 37°C and a pH of 7.2, had a 44,789 bp linear double-strand DNA genome, and within the genome sequence, there are 61 genes, all of which are encoded into proteins. Although this bacteriophage does not have any virulence factors or antimicrobial resistance genes and had specific lytic activity against some antimicrobial resistance S. aureus clinical isolates.

Keywords

phage Rih21; MRSA; novel bacteriophage; S. aureus; bacteriophage; phage genome analysis

Subject

Biology and Life Sciences, Virology

Comments (1)

Comment 1
Received: 5 October 2022
Commenter: Arif Ghayyib
Commenter's Conflict of Interests: Author
Comment: The introduction has been updated
Images have been added with clearer resolution
The result of antibiotic Sensitivity stated
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