Version 1
: Received: 24 August 2022 / Approved: 30 August 2022 / Online: 30 August 2022 (04:09:57 CEST)
How to cite:
Alamir, A.; Watterson, J.; Attafi, I. Development and Validation of UPLC-Qtof-MS Method for Blood Analysis of Amphetamine-Related Drug Isomers. Preprints2022, 2022080504. https://doi.org/10.20944/preprints202208.0504.v1
Alamir, A.; Watterson, J.; Attafi, I. Development and Validation of UPLC-Qtof-MS Method for Blood Analysis of Amphetamine-Related Drug Isomers. Preprints 2022, 2022080504. https://doi.org/10.20944/preprints202208.0504.v1
Alamir, A.; Watterson, J.; Attafi, I. Development and Validation of UPLC-Qtof-MS Method for Blood Analysis of Amphetamine-Related Drug Isomers. Preprints2022, 2022080504. https://doi.org/10.20944/preprints202208.0504.v1
APA Style
Alamir, A., Watterson, J., & Attafi, I. (2022). Development and Validation of UPLC-Qtof-MS Method for Blood Analysis of Amphetamine-Related Drug Isomers. Preprints. https://doi.org/10.20944/preprints202208.0504.v1
Chicago/Turabian Style
Alamir, A., James Watterson and Ibraheem Attafi. 2022 "Development and Validation of UPLC-Qtof-MS Method for Blood Analysis of Amphetamine-Related Drug Isomers" Preprints. https://doi.org/10.20944/preprints202208.0504.v1
Abstract
Abuse of amphetamine-related drugs (ARDs) causes traffic accidents, violence, and overdose. In forensic toxicology, analysis for ARDs in biological samples can help identify those driving or performing other tasks under the influence of drugs, clarify the cause of death, and identify recent drug users. In this study, we validated a pseudo-isocratic UPLC-qTOF-MS method following mixed mode cation exchange (MMSPE) extraction for analysis of ARDs in blood. The procedure requires 250 μL of blood to achieve a limit of quantification (LOQ) and detection (LOD) of 20 ng/mL for all analytes. In aged animal blood samples, extraction recoveries of 63-90% and matrix effects of 9-21% were observed. Precision and accuracy for all analytes were within 20% and 89–118%, respectively. The analytical method was developed and validated in accordance with the Scientific Working Group for Forensic Toxicology (SWGTOX) Standard. It has acceptable accuracy and precision for use in doping control and forensic toxicology.
Medicine and Pharmacology, Pharmacology and Toxicology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
