Version 1
: Received: 3 August 2022 / Approved: 8 August 2022 / Online: 8 August 2022 (13:46:09 CEST)
How to cite:
Fatemi, R.; Fathimoghadam, H.; Ghadiri, A.; Farbod, Y. Effects of Crocin on Brain Neurotrophins, Cognition, Sensory and Motor Dysfunction in Rats Demyelination with Ethidium Bromide. Preprints2022, 2022080161. https://doi.org/10.20944/preprints202208.0161.v1
Fatemi, R.; Fathimoghadam, H.; Ghadiri, A.; Farbod, Y. Effects of Crocin on Brain Neurotrophins, Cognition, Sensory and Motor Dysfunction in Rats Demyelination with Ethidium Bromide. Preprints 2022, 2022080161. https://doi.org/10.20944/preprints202208.0161.v1
Fatemi, R.; Fathimoghadam, H.; Ghadiri, A.; Farbod, Y. Effects of Crocin on Brain Neurotrophins, Cognition, Sensory and Motor Dysfunction in Rats Demyelination with Ethidium Bromide. Preprints2022, 2022080161. https://doi.org/10.20944/preprints202208.0161.v1
APA Style
Fatemi, R., Fathimoghadam, H., Ghadiri, A., & Farbod, Y. (2022). Effects of Crocin on Brain Neurotrophins, Cognition, Sensory and Motor Dysfunction in Rats Demyelination with Ethidium Bromide. Preprints. https://doi.org/10.20944/preprints202208.0161.v1
Chicago/Turabian Style
Fatemi, R., Atatalah Ghadiri and Yaghoub Farbod. 2022 "Effects of Crocin on Brain Neurotrophins, Cognition, Sensory and Motor Dysfunction in Rats Demyelination with Ethidium Bromide" Preprints. https://doi.org/10.20944/preprints202208.0161.v1
Abstract
Objective: The purpose of this study was to investigate the effects of Crocin on brain neuroterophins, cognition, sensory and motor dysfunction and compare to fingolimod effects in experimental model of demyelination with Ethidium Bromide EB in female Wistar rats. Methods: Animals were assigned in to 8 groups; Sham, Sham operated (ShOp), EAE, crocin treated (Cr5,10,20 mg/kg), Vehicle, Fingolompd (Fing) and fingolimod + crocin (Cr+Fing). Demyelination was induced by single dose injection of 10 μl of EB 0.1% into the fourth ventricle of the brain. Crocin and fingolimod were applied for 21 days, daily, oral gavage. BDNF, NGF1, nerve conduction velocities, tail flake latency, balance and behavioral variables were sampled and analyzed by paired t-test and ANOVA test with repeated post hoc measurements. Results: The results showed that crocin improves all studied factors, but remarkable imrovments were observed in dosage of 10 mg/kg. Crocin (10mg/kg) and fingolimod (1mg/kg) significantly improved cognition variables in open field test, sensory and motor nerve conduction velocity, tail flick latency and clinical signs (p<005). In addition, applying of crocin co-administered with fingolimod led to significant increases in all assessed factors, greater than crocin or fingolimod intervention alone (α≤0.001). Conclusion: Based on the current findings, crocin can improve the level of brain neurotrophins, exploratory behavior and nerve conduction after demyelination as close as fingolimod results. So, crocins can be considered as a neuro supportive agent in the management of degenerative diseases maybe similar to fingolimod mechanism.
Medicine and Pharmacology, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
