Version 1
: Received: 29 July 2022 / Approved: 1 August 2022 / Online: 1 August 2022 (04:28:27 CEST)
How to cite:
Rull, V.; Sacristán-Soriano, O.; Sànchez-Melsió, A.; Borrego, C.; Vegas-Vilarrúbia, T. Bacterial Phylogenetic Markers in Lake Sediments as Evidence for Historical Hemp Retting. Preprints2022, 2022080003. https://doi.org/10.20944/preprints202208.0003.v1
Rull, V.; Sacristán-Soriano, O.; Sànchez-Melsió, A.; Borrego, C.; Vegas-Vilarrúbia, T. Bacterial Phylogenetic Markers in Lake Sediments as Evidence for Historical Hemp Retting. Preprints 2022, 2022080003. https://doi.org/10.20944/preprints202208.0003.v1
Rull, V.; Sacristán-Soriano, O.; Sànchez-Melsió, A.; Borrego, C.; Vegas-Vilarrúbia, T. Bacterial Phylogenetic Markers in Lake Sediments as Evidence for Historical Hemp Retting. Preprints2022, 2022080003. https://doi.org/10.20944/preprints202208.0003.v1
APA Style
Rull, V., Sacristán-Soriano, O., Sànchez-Melsió, A., Borrego, C., & Vegas-Vilarrúbia, T. (2022). Bacterial Phylogenetic Markers in Lake Sediments as Evidence for Historical Hemp Retting. Preprints. https://doi.org/10.20944/preprints202208.0003.v1
Chicago/Turabian Style
Rull, V., Carles Borrego and Teresa Vegas-Vilarrúbia. 2022 "Bacterial Phylogenetic Markers in Lake Sediments as Evidence for Historical Hemp Retting" Preprints. https://doi.org/10.20944/preprints202208.0003.v1
Abstract
Documenting prehistoric and historical hemp retting for fiber extraction is important in the study of human uses of this iconic plant and its cultural implications. In paleoecology, hemp retting is usually inferred from indirect proxies, notably anomalously high percentages of Cannabis pollen in lake sediments, but some recent studies have also used specific molecular biomarkers (cannabinol, Cannabis DNA) as a more straightforward evidence. Here we provide direct evidence of hemp retting by identifying phylogenetic signatures (16S rRNA genes) from pectinolytic bacteria actually responsible for the fermentation process that separates the fiber from the stalk, namely Bacillus, Clostridium, Escherichia, Massilia, Methylobacterium, Pseusomonas, Rhizobium and Rhodobacter. These analyses have been performed in the sediments from an Iberian lake previously considered as an important hemp retting site during the last five centuries, on the basis of Cannabis pollen abundances. The good match between biomarker and pollen evidence, in the context of the recent historical development of hemp industry in Spain, can be useful to interpret paleoecological records from other similar lakes in the way toward a more regional view on the introduction, spreading, uses and associated cultural connotations of Cannabis in the Iberian Peninsula within European and Mediterranean contexts.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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