Version 1
: Received: 13 June 2022 / Approved: 14 June 2022 / Online: 14 June 2022 (16:21:47 CEST)
How to cite:
Araujo, I. L.; Piraine, R. E. A.; Fischer, G.; Leite, F. P. L. Recombinant BoHV-5 Glycoprotein Elicits Long-Lasting Protective Immunity in Cattle. Preprints2022, 2022060207. https://doi.org/10.20944/preprints202206.0207.v1
Araujo, I. L.; Piraine, R. E. A.; Fischer, G.; Leite, F. P. L. Recombinant BoHV-5 Glycoprotein Elicits Long-Lasting Protective Immunity in Cattle. Preprints 2022, 2022060207. https://doi.org/10.20944/preprints202206.0207.v1
Araujo, I. L.; Piraine, R. E. A.; Fischer, G.; Leite, F. P. L. Recombinant BoHV-5 Glycoprotein Elicits Long-Lasting Protective Immunity in Cattle. Preprints2022, 2022060207. https://doi.org/10.20944/preprints202206.0207.v1
APA Style
Araujo, I. L., Piraine, R. E. A., Fischer, G., & Leite, F. P. L. (2022). Recombinant BoHV-5 Glycoprotein Elicits Long-Lasting Protective Immunity in Cattle. Preprints. https://doi.org/10.20944/preprints202206.0207.v1
Chicago/Turabian Style
Araujo, I. L., Geferson Fischer and Fábio Pereira Leivas Leite. 2022 "Recombinant BoHV-5 Glycoprotein Elicits Long-Lasting Protective Immunity in Cattle" Preprints. https://doi.org/10.20944/preprints202206.0207.v1
Abstract
Bovine herpesvirus (BoHV)-5 is a worldwide distributed pathogen usually associated with a lethal neurological disease (meningoencephalitis) in dairy and beef cattle resulting in important economic losses due to the cattle industry. Using recombinant glycoprotein D of BoHV-5 (rgD5), we evaluated the long-duration humoral immunity of the recombinant vaccines in a cattle model. Here we report that two doses of intramuscular immunization, particularly with the rgD5ISA vaccine, are superior to iBoHV-5ISA immunization in the induction of long-lasting antibody responses. Recombinant gD5 antigen elicited tightly mRNA transcription of the Bcl6 and the chemokine receptor CXCR5 which mediate memory B cells and long-lived plasma cells in germinal centers (GCs). In addition, using an in-house Enzyme-Linked Immunosorbent Assay (ELISA) we observed higher and earlier responses of rgD5-specific IgG antibody and the upregulation of mRNA transcription of IL2, IL4, IL10, IL15 and IFN-γ cytokines in rgD5 vaccinated cattle, indicating a mixed immune response. We further show that rgD5 immunization provides protection against both BoHV -1 and -5. Our findings indicate that the rgD5-based vaccine represents an effective vaccine strategy to induce an efficient control of alpha-herpesviruses.
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.