Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Altered RNome expression in Murine Gastrocnemius Muscle Following Exposure to Jararhagin, a Metalloproteinase from Bothrops Jararaca Venom

Version 1 : Received: 1 June 2022 / Approved: 6 June 2022 / Online: 6 June 2022 (03:56:53 CEST)

A peer-reviewed article of this Preprint also exists.

Nascimento, A.; Zychar, B.C.; Pessôa, R.; da Silva Duarte, A.J.; Clissa, P.B.; Sanabani, S.S. Altered RNome expression in Murine Gastrocnemius Muscle following Exposure to Jararhagin, a Metalloproteinase from Bothrops jararaca Venom. Toxins 2022, 14, 472. Nascimento, A.; Zychar, B.C.; Pessôa, R.; da Silva Duarte, A.J.; Clissa, P.B.; Sanabani, S.S. Altered RNome expression in Murine Gastrocnemius Muscle following Exposure to Jararhagin, a Metalloproteinase from Bothrops jararaca Venom. Toxins 2022, 14, 472.

Journal reference: Toxins 2022, 14, 472
DOI: 10.3390/toxins14070472

Abstract

Small RNAs (sRNA) and microRNAs (miRNAs) are small endogenous noncoding single-stranded RNAs that regulate gene expression in eukaryotes. Experiments in mice and humans have revealed that a typical small RNA can affect the expression of a wide range of genes, implying that small RNAs function as global regulators. Here, we used small RNA deep sequencing to investigate at how jararhagin, a metalloproteinase toxin produced from the venom of Bothrops jararaca, affected mmu-miRs expression in mice 2 h and 24 h after injection. The findings revealed that seven mmu-miRs were substantially differentially expressed (p-value (p (Corr) cut-off 0.05, FC 2) at 2h after jararhagin exposure, and that the majority of them were upregulated when compared to PBS. In contrast to these findings, a comparison of Jar 24h vs PBS 24hrs demonstrated that the majority of identified mmu-miRs were downregulated. Furthermore, the studies demonstrated that mmu-miR can target the expression of several genes involved in the MAPK signaling pathway. The steady antithetical regulation of mmu-miRs may correlates with the expression of genes that trigger apoptosis via MAPK in the early stages, and this effect intensifies with time. The findings expand our understanding of the effects of jararhagin on local tissue lesions at the molecular level.

Keywords

Small RNAs; Jararhagin; Venom

Subject

MEDICINE & PHARMACOLOGY, Other

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