Customizable Collagen Vitrigel Scaffolds and Preliminary Results in Corneal Engineering

María Dolores Montalvo-Parra; Wendy Ortega-Lara; Denise Loya-García; Andres Bustamante-Arias; Guillermo Isaac Guerrero-Ramírez; César E. Calzada-Rodríguez; Guiomar Farid Torres-Guerrero; Bestabé Hernández-Sedas; Italia Tatnaí Cárdenas-Rodríguez; Sergio E. Guevara-Quintanilla; Marcelo Salán-Flores; Miguel Angel Hernández-Delgado; Salvador Garza-González; Mayra G Gamboa-Quintanilla; Luis Guillermo Villagómez-Valdez; Judith Zavala; Jorge Eugenio Valdez-García

doi:10.20944/preprints202205.0396.v1

Submit preprint
How it Works
Instructions for Authors
Subject Areas
Advisory Board
screening preprints
About
Awards
Log in/Register

Instructions for Authors
Awards
About
FAQ
Submit Log in/Register

Share this article with

Create alert

Email:

Load new image

preprints.org > chemistry and materials science > biomaterials > doi: 10.20944/preprints202205.0396.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Customizable Collagen Vitrigel Scaffolds and Preliminary Results in Corneal Engineering

María Dolores Montalvo-Parra

Wendy Ortega-Lara

Denise Loya-García

Andres Bustamante-Arias

Guillermo Isaac Guerrero-Ramírez

César E. Calzada-Rodríguez

Guiomar Farid Torres-Guerrero

Bestabé Hernández-Sedas

Italia Tatnaí Cárdenas-Rodríguez

Sergio E. Guevara-Quintanilla

Marcelo Salán-Flores

Miguel Angel Hernández-Delgado

Salvador Garza-González

Mayra G Gamboa-Quintanilla

Luis Guillermo Villagómez-Valdez

Judith Zavala

^* ,

Jorge Eugenio Valdez-García

Version 1 : Received: 28 May 2022 / Approved: 30 May 2022 / Online: 30 May 2022 (11:25:19 CEST)

A peer-reviewed article of this Preprint also exists.

Montalvo-Parra, M.D.; Ortega-Lara, W.; Loya-García, D.; Bustamante-Arias, A.; Guerrero-Ramírez, G.-I.; Calzada-Rodríguez, C.E.; Torres-Guerrero, G.F.; Hernández-Sedas, B.; Cárdenas-Rodríguez, I.T.; Guevara-Quintanilla, S.E.; Salán-Gomez, M.; Hernández-Delgado, M.Á.; Garza-González, S.; Gamboa-Quintanilla, M.G.; Villagómez-Valdez, L.G.; Zavala, J.; Valdez-García, J.E. Customizable Collagen Vitrigel Membranes and Preliminary Results in Corneal Engineering. Polymers 2022, 14, 3556. Montalvo-Parra, M.D.; Ortega-Lara, W.; Loya-García, D.; Bustamante-Arias, A.; Guerrero-Ramírez, G.-I.; Calzada-Rodríguez, C.E.; Torres-Guerrero, G.F.; Hernández-Sedas, B.; Cárdenas-Rodríguez, I.T.; Guevara-Quintanilla, S.E.; Salán-Gomez, M.; Hernández-Delgado, M.Á.; Garza-González, S.; Gamboa-Quintanilla, M.G.; Villagómez-Valdez, L.G.; Zavala, J.; Valdez-García, J.E. Customizable Collagen Vitrigel Membranes and Preliminary Results in Corneal Engineering. Polymers 2022, 14, 3556.

Abstract

We set a feasible method to produce tailored collagen scaffolds and analyzed its potential for corneal engineering. Collagen-vitrigel membranes (CVM) were produced with a 1:1 ratio of Dulbecco’s Modified Eagle’s medium (DMEM), 1% antibiotics and 8% fetal bovine serum, and 5mg/mL collagen type I. Three volumes of collagen were used: 1X (2.8 L/mm2 of collagen), 2X, and 3X. Vitrification was done at 40% relative humidity (RH), 40° C, and 30 rpm using a matryoshka system set with a shaking-oven and a desiccator with a saturated K2CO3 solution. The CVM was characterized for width, microstructure, transparency, and biocompatibility using NIH3T3 cells. Surgical manipulation was assessed in an ex vivo corneal model. Constructs of corneal endothelial cells (CECs) and 2X-CVM were transplanted into five 18-month-old White New Zealand rabbits. CVM exhibited homogeneous surface and laminar organization. Membrane width increased with gel volume from 3.65µm to 7.2µm. 1X and 2X-CVM exhibited a 99% transmittance. NIH3T3 cells concentration increased 3-fold within 48 h with no significant difference among the 3 CVM (p = 0.323). The 2X-CVM was surgically manipulable. Transplantation of corneal endothelial cells (CECs) seeded over 2X-CVM restored corneal endothelium. The matrioshka system is a feasible method that yields CVM suitable for corneal engineering.

Keywords

scaffold; collagen vitrigel; tissue engineering; cornea; corneal endothelium

Subject

Chemistry and Materials Science, Biomaterials

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

Not displayed online.

Importance: How significant is the paper to the field?

Outstanding/highlight paper

Significant contribution

Incremental contribution

No contribution

I am not qualified to judge

Soundness of evidence/arguments presented:

Conclusions well supported

Most conclusions supported (minor revision needed)

Incomplete evidence (major revision needed)

Hypothesis, unsupported conclusions, or proof-of-principle

I am not qualified to judge

Please leave your comment to this article below.

Mathematical equations can be typed in either LaTeX formats \\[ ... \\] or $$ ... $$, or MathML format <math> ... </math>. Try the LaTeX or MathML example.

Type equation:

Preview:

Copy equation into message below

Close menu

Please click a symbol to insert it into the message box below:

Please enter the link here:

Please enter a valid URL

Optionally, you can enter text that should appear as linked text:

Copy link into message below

Close menu

Please enter or paste the URL to the image here (please only use links to jpg/jpeg, png and gif images):

Please enter a valid URL

Copy image into message below

Close menu

Type author name or keywords to filter the list of references in this group (you can add a new citation under Bibliography):

No existing citations in Discussion Group

Wikify editor is a simple editor for wiki-style mark-up. It was written by MDPI for Sciforum in 2014. The rendering of the mark-up is based on Wiky.php with some tweaks. Rendering of mathematical equations is done with MathJax. Please send us a message for support or for reporting bugs.

Close menu

Please declare any conflicts of interest you may have with this paper, financial or otherwise.

I do not have a conflict of interest

I would like to declare a conflict of interest

Display my name on the website Send my name and email address to the authors

Captcha

Renew

I accept the following conditions:

Comments must follow the standards of professional discourse and should focus on the scientific content of the article. Insulting or offensive language, personal attacks and off-topic remarks will not be permitted. Comments must be written in English. Preprints reserves the right to remove comments without notice. Readers who post comments are obliged to declare any competing interests, financial or otherwise.

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)

* All users must log in before leaving a comment

what’s this?

Add a record of this review to Publons to track and showcase your reviewing expertise across the world’s journals.

Downloads 0

Comments 0

Metrics 0

Get PDF Cite Share 0

Bookmark BibSonomy Mendeley Reddit Delicious

Alerts

Notify me about updates to this article or when a peer-reviewed version is published.

Preprints.org is a free preprint server subsidized by MDPI in Basel, Switzerland.

MDPI Initiatives

SciProfiles
Sciforum
Encyclopedia
MDPI Books
Scilit
Proceedings
JAMS

Important links

How it Works
Advisory Board
FAQ
Friendly Journals
Instructions for Authors
About
Statistics

Choose the area that interest you and we will send you notifications of new preprints at your preferred frequency.

Disclaimer

We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.

RSS feed for

Preprints: The Multidisciplinary Preprint Platform

Paste the URL in your RSS reader:

https://www.preprints.org/rss

If you want to subscribe our website in subject-wide, you could find the URL by choosing in the following:

https://www.preprints.org/rss

Please note that by using ScienceDirect RSS feeds, you agree to our Terms of Use and Privacy Policy.