Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Transcriptome Analysis Reveals Hepatotoxicity in Zebrafish Induced by Cyhalofop-Butyl

Version 1 : Received: 4 April 2022 / Approved: 6 April 2022 / Online: 6 April 2022 (08:33:09 CEST)

How to cite: Zhou, T.; Liu, J.; Yang, Y.; Liu, T. Transcriptome Analysis Reveals Hepatotoxicity in Zebrafish Induced by Cyhalofop-Butyl. Preprints 2022, 2022040036 (doi: 10.20944/preprints202204.0036.v1). Zhou, T.; Liu, J.; Yang, Y.; Liu, T. Transcriptome Analysis Reveals Hepatotoxicity in Zebrafish Induced by Cyhalofop-Butyl. Preprints 2022, 2022040036 (doi: 10.20944/preprints202204.0036.v1).

Abstract

Cyhalofop-butyl is a highly effective aryloxyphenoxypropionate herbicide and widely used for weed control in paddy fields. With the increasing residue of herbicides, it poses a threat to the survival of aquatic organisms. Here, we evaluated the effect of cyhalofop-butyl on zebrafish to explore its potential hepatotoxic mechanism. The results showed that cyhalofop-butyl (0.1, 0.2 and 0.4 mg/L) induced hepatocyte degeneration, vacuolation and necrosis of larvae after embryonic exposure for 4 days and caused liver atrophy after exposure for 5 days. Meanwhile, the activities of enzymes related to liver function named alanine transaminase (ALT), aspartate transaminase (AST) were significantly increased by 0.2 mg/L cyhalofop-butyl and higher. And the contents of triglyceride (TG) involved in lipid metabolism was significantly decreased by 0.4 mg/L cyhalofop-buty. The effects of cyhalofop-butyl on zebrafish larvae were further demonstrated by GO (Gene Ontology) and KEGG pathway analysis. Cyhalofop-butyl (0.1, 0.2, 0.4 mg/L) altered the expression of 116, 154, 397 genes in liver,these genes are mainly enriched in metabolism (such as lipid metabolism, amino acid metabolism), immune system (Toll-like receptor signaling pathway) and endocrine system (PPAR signaling pathway). Furthermore, the expression of key genes related to liver development combined with RNA-Seq results, indicated that cyhalofop-butyl might damage the liver development of zebrafish larvae and cause metabolic disorders. To sum up, our research results reveal the physiological and molecular responses of zebrafish liver to cyhalofop-butyl and provide new insights for further studying the mechanism of cyhalofop-butyl toxicity to aquatic organisms.

Keywords

Cyhalofop-butyl; Zebrafish; Liver development; Hepatotoxic; RNA-Seq

Subject

EARTH SCIENCES, Environmental Sciences

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