Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

A Non-Invasive Neonatal Signature Predicts Later Development of Atopic Diseases

Version 1 : Received: 19 March 2022 / Approved: 22 March 2022 / Online: 22 March 2022 (07:33:48 CET)

A peer-reviewed article of this Preprint also exists.

Sereme, Y.; Michel, M.; Mezouar, S.; Guindo, C.O.; Kaba, L.; Grine, G.; Mura, T.; Mège, J.-L.; Tran, T.A.; Corbeau, P.; Filleron, A.; Vitte, J. A Non-Invasive Neonatal Signature Predicts Later Development of Atopic Diseases. J. Clin. Med. 2022, 11, 2749. Sereme, Y.; Michel, M.; Mezouar, S.; Guindo, C.O.; Kaba, L.; Grine, G.; Mura, T.; Mège, J.-L.; Tran, T.A.; Corbeau, P.; Filleron, A.; Vitte, J. A Non-Invasive Neonatal Signature Predicts Later Development of Atopic Diseases. J. Clin. Med. 2022, 11, 2749.

Journal reference: J. Clin. Med. 2022, 11, 2749
DOI: 10.3390/jcm11102749

Abstract

Background: Preterm birth is a major cause of morbidity and mortality in infants and children. Non-invasive methods for screening the neonatal immune status are lacking. Archaea, a prokaryotic life domain, comprise methanogenic species that are part of the neonatal human microbiota and contribute to early immune imprinting. However, they have not yet been characterized in preterm neonates. Objective: To characterize the gut immunological and methanogenic Archaeal (MA) signature in preterm neonates, using the presence or absence of atopic conditions at the age of 1 year as a clinical endpoint. Methods: Meconium and stool were collected from preterm neonates and used to develop a standardized stool preparation method for the assessment of mediators and cytokines and characterize the qPCR kinetics of gut MA. Analysis addressed the relationship between immunological biomarkers, Archaea abundance, and atopic disease at age 1. Results: Immunoglobulin E, tryptase, calprotectin, EDN, cytokines, and MA were detectable in the meconium and later samples. Atopic conditions at age 1 year were positively associated with neonatal EDN, IL-1β, IL-10, IL-6, and MA abundance. The latter was negatively associated with neonatal EDN, IL-1β and IL-6. Conclusion: We report a non-invasive method for establishing a gut immunological and Archaeal signature in preterm neonates, predictive of atopic diseases at the age of 1 year

Keywords

preterm birth; fecal mediator and cytokine; methanogenic Archaea; allergy; atopy

Subject

BIOLOGY, Other

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