Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Population Genetic Structure of the Bean Leaf Beetle Ootheca mutabilis (Coleoptera: Chrysomelidae) in Uganda

Dalton Kanyesigye
* ORCID logo ,
Vincent Pius Alibu
,
Wee Tek Tay
ORCID logo ,
Polycarp Nalela
,
Pamela Paparu
,
Samuel Olaboro
,
Stanley Tamusange Nkalubo
ORCID logo ,
Ismail Siraj Kayondo
,
Gonçalo Silva
ORCID logo ,
Susan Elizabeth Seal
,
Michael Hilary Otim
* ORCID logo
Version 1 : Received: 5 March 2022 / Approved: 14 March 2022 / Online: 14 March 2022 (16:48:57 CET)

A peer-reviewed article of this Preprint also exists.

Kanyesigye, D.; Alibu, V.P.; Tay, W.T.; Nalela, P.; Paparu, P.; Olaboro, S.; Nkalubo, S.T.; Kayondo, I.S.; Silva, G.; Seal, S.E.; Otim, M.H. Population Genetic Structure of the Bean Leaf Beetle Ootheca mutabilis (Coleoptera: Chrysomelidae) in Uganda. Insects 2022, 13, 543. Kanyesigye, D.; Alibu, V.P.; Tay, W.T.; Nalela, P.; Paparu, P.; Olaboro, S.; Nkalubo, S.T.; Kayondo, I.S.; Silva, G.; Seal, S.E.; Otim, M.H. Population Genetic Structure of the Bean Leaf Beetle Ootheca mutabilis (Coleoptera: Chrysomelidae) in Uganda. Insects 2022, 13, 543.

Abstract

leaf beetle (BLB) (Ootheca mutabilis) has emerged as an important bean pest in Uganda, leading to devastating crop losses. There is limited information on the population genetic structure of BLB despite their importance. In this study, novel microsatellite markers and the partial mitochondrial cytochrome oxidase subunit I (mtCOI) gene sequences were used to analyze the spatial population genetic structure, genetic differentiation, gene flow and haplotype diversity of 87 O. mutabilis samples from five populations. We identified 19,356 simple sequence repeats (SSRs) (mono, di, tri, tetra, penta, and hexa-nucleotides) of which 81 di, tri and tetra-nucleotides were selected for primer synthesis. Five highly polymorphic SSR markers (4-21 alleles, heterozygosi-ty 0.59-0.84, polymorphic information content (PIC) 50.13-83.14%) were used for this study. Analyses of the five O. mutabilis populations with these five novel SSRs found 89% of genetic variation occurring within individuals, 9% among individuals and 2% among populations. Genetic differentiation was low but significant for SSR and insignificant for mtCOI partial sequence data while gene flow was high across the populations. There was no evidence of isolation by distance between geographical and genetic distances. Bayesian clustering identified signature of admixture that suggests genetic contributions from two ancestral genetic lineages, and the median-joining haplotype network showed low differentiation of many different haplotypes from the most common haplotype. Low genetic differentiation and high gene flow indicates unrestricted migrations between populations. This information will contribute to the design of BLB control strategies.

Keywords

genetic differentiation; leaf beetle; mitochondrial DNA; microsatellites; haplotype; gene flow

Subject

Biology and Life Sciences, Insect Science

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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