Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Dioscin-mediated Autophagy Alleviates Neuronal Degeneration in MPP+-treated SH-SY5Y of Parkinson's Disease Model

Version 1 : Received: 26 February 2022 / Approved: 3 March 2022 / Online: 3 March 2022 (10:36:29 CET)

How to cite: Azam, S.; Haque, M.E.; Cho, D.; Kim, J.; Jakaria, M.; Kim, I.; Choi, D. Dioscin-mediated Autophagy Alleviates Neuronal Degeneration in MPP+-treated SH-SY5Y of Parkinson's Disease Model. Preprints 2022, 2022030063 (doi: 10.20944/preprints202203.0063.v1). Azam, S.; Haque, M.E.; Cho, D.; Kim, J.; Jakaria, M.; Kim, I.; Choi, D. Dioscin-mediated Autophagy Alleviates Neuronal Degeneration in MPP+-treated SH-SY5Y of Parkinson's Disease Model. Preprints 2022, 2022030063 (doi: 10.20944/preprints202203.0063.v1).

Abstract

Autophagy is a cellular homeostatic process by which cells degrade and recycle their malfunctioned contents, and impairment in this process leads to Parkinson's disease (PD) pathogenesis. Dioscin, a steroidal saponin, has induced autophagy in several cell lines and animal models. The role of dioscin-mediated autophagy in PD remains to be investigated. Therefore, this study aims to investigate the hypothesis that dioscin-regulated autophagy and autophagy-related genes (ATG) could protect neuronal cells in PD via reducing apoptosis and enhancing neurogenesis. In this study, the 1-methyl-4-phenylpyridinium ion (MPP+) were used to induce neurotoxicity and impair autophagic flux in a human neuroblastoma cell line (SH-SY5Y). The result showed that dioscin pre-treatment counters MPP+-mediated autophagic flux impairment and alleviates MPP+-induced apoptosis by downregulating activated caspase-3 and Bax expression while increasing Bcl-2 expression. In addition, dioscin pre-treatment was found to increase neurotrophic factors and tyrosine hydroxylase expression, suggesting that dioscin could ameliorate MPP+-induced degeneration in dopaminergic neurons and benefit the PD model. Interestingly, the neuroprotective activities of dioscin were suppressed when co-treated with chloroquine (CQ), an autophagosome-lysosome inhibitor. In summary, we showed dioscin’s neuroprotective activity in neuronal SH-SY5Y cells might be partly related to its autophagy induction and suppression of the mitochondrial apoptosis pathway.

Keywords

MPP+; neurotoxicity; autophagy; apoptosis; neuroprotection; Parkinson's disease

Subject

MEDICINE & PHARMACOLOGY, Pharmacology & Toxicology

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