Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Modulation of Metabolic Hormone Signaling via a Circadian Hormone and Biogenic Amine in Drosophila melanogaster

Version 1 : Received: 23 February 2022 / Approved: 25 February 2022 / Online: 25 February 2022 (09:27:26 CET)

A peer-reviewed article of this Preprint also exists.

Braco, J.T.; Nelson, J.M.; Saunders, C.J.; Johnson, E.C. Modulation of Metabolic Hormone Signaling via a Circadian Hormone and Biogenic Amine in Drosophila melanogaster. Int. J. Mol. Sci. 2022, 23, 4266. Braco, J.T.; Nelson, J.M.; Saunders, C.J.; Johnson, E.C. Modulation of Metabolic Hormone Signaling via a Circadian Hormone and Biogenic Amine in Drosophila melanogaster. Int. J. Mol. Sci. 2022, 23, 4266.

Journal reference: Int. J. Mol. Sci. 2022, 23, 4266
DOI: 10.3390/ijms23084266

Abstract

In insects, Adipokinetic hormone is the primary hormone responsible for the mobilization of stored energy. While a growing body of evidence has solidified role of Adipokinetic hormone (AKH) in modulating the physiological and behavioral responses to metabolic stress, little is known about the upstream endocrine circuit that directly regulates AKH release. We evaluated the AKH-producing cell (APC) transcriptome to identify potential regulatory elements controlling APC activity, and found that a number of receptors show consistent expression levels, including all known dopamine receptors and the Pigment Dispersing Factor receptor (PDFR). We tested the consequences of targeted genetic knockdown and found that AKH expression of RNAi elements corresponding to each dopamine receptor caused a significant reduction in survival under starvation. In contrast, PDFR knockdown significantly extended lifespan under starvation whereas expression of a tethered PDF in APCs resulted in a significantly shorter lifespan. These manipulations caused various changes in locomotor activity under starvation. We used live-cell imaging to evaluate the acute effects of the ligands for these receptors on APC activation. Dopamine application led to a transient increase in intracellular calcium in a sugar-dependent manner. Furthermore, co-application of dopamine and ecdysone led to a complete loss of this response, suggesting that these two hormones act antagonistically. We also found that PDF application led to an increase in cAMP in APCs, and that this response was dependent on expression of the PDFR in APCs. Together these results suggest a complex circuit in which multiple hormones act on APCs to modulate metabolic state.

Keywords

Metabolism; Adipokinetic Hormone; Pigment Dispersing Factor, Dopamine, Ecdysone, Signaling

Subject

BIOLOGY, Other

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