Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

miR-221-3p/222-3p Cluster Expression in Human Adipose Tissue is Related to Obesity and Type 2 Diabetes

Adriana-Mariel Gentile
ORCID logo ,
Said Lhamyani
ORCID logo ,
Mercedes Clemente-Postigo
,
Enrique Estepa
,
Maria Mengual-Mesa
,
Francisco J. Bermúdez-Silva
ORCID logo ,
Alberto Rodríguez-Cañete
,
Francisco J. Tinahones
,
Gabriel Olveira
,
Rajaa El Bekay
* ORCID logo
Version 1 : Received: 23 February 2022 / Approved: 24 February 2022 / Online: 24 February 2022 (09:54:33 CET)

A peer-reviewed article of this Preprint also exists.

Gentile, A.-M.; Lhamyani, S.; Mengual-Mesa, M.; García-Fuentes, E.; Bermúdez-Silva, F.-J.; Rojo-Martínez, G.; Clemente-Postigo, M.; Rodriguez-Cañete, A.; Olveira, G.; El Bekay, R. MiR-221-3p/222-3p Cluster Expression in Human Adipose Tissue Is Related to Obesity and Type 2 Diabetes. Int. J. Mol. Sci. 2023, 24, 17449. Gentile, A.-M.; Lhamyani, S.; Mengual-Mesa, M.; García-Fuentes, E.; Bermúdez-Silva, F.-J.; Rojo-Martínez, G.; Clemente-Postigo, M.; Rodriguez-Cañete, A.; Olveira, G.; El Bekay, R. MiR-221-3p/222-3p Cluster Expression in Human Adipose Tissue Is Related to Obesity and Type 2 Diabetes. Int. J. Mol. Sci. 2023, 24, 17449.

Abstract

Background: The course of obesity and type 2 diabetes (T2D) development is highly dependent on adipose tissue (AT) angiogenesis. Moreover, angiogenic microRNAs (miRNAs) play pivotal role in AT functionality. The aim of this study was to analyze the relationship of the human AT miR-221-3p/222-3p cluster and their regulatory network with obesity and T2D. Methods: miR-221-3p/222-3p and their target genes (TG) expression levels were measured in visceral and subcutaneous ATs from patients classified according to their BMI and to their glycemic status with a high degree of insulin resistance (IR) and T2D. In silico analyses of miR-221-3p/222-3p and their TGs were performed to identify relevant signaling pathways. Results: A multivariate analysis, including the simultaneous expression of miR-221-3p and miR-222-3p as dependent variables, showed significant differences considering the variables; tissue depot, obesity, IR and T2D altogether as independent variables. In addition, miRNAs and their TGs were differentially expressed according to obesity degree, glycemic status, and AT depot type. Our in silico analysis showed that miR-221-3p/222-3p cluster TGs are mostly involved in angiogenesis, WNT signaling pathway and apoptosis. Conclusion: These findings suggest that the miR-221-3p/222-3p cluster and their related regulatory networks could represent tangible targets for the management of obesity and associated metabolic disorders

Keywords

miR-221-3p/222-3p cluster; human adipose tissue; obesity; type 2 diabetes

Subject

Medicine and Pharmacology, Endocrinology and Metabolism

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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