Version 1
: Received: 17 February 2022 / Approved: 18 February 2022 / Online: 18 February 2022 (10:01:58 CET)
How to cite:
Khashaveh, A.; An, X.; Shan, S.; Pang, X.; Li, Y.; Fu, X.; Zhang, Y. The microRNAs in the Antennae of Apolygus lucorum (Hemiptera: Miridae): Expression Properties and Targets Prediction. Preprints.org2022, 2022020232. https://doi.org/10.20944/preprints202202.0232.v1.
Khashaveh, A.; An, X.; Shan, S.; Pang, X.; Li, Y.; Fu, X.; Zhang, Y. The microRNAs in the Antennae of Apolygus lucorum (Hemiptera: Miridae): Expression Properties and Targets Prediction. Preprints.org 2022, 2022020232. https://doi.org/10.20944/preprints202202.0232.v1.
Cite as:
Khashaveh, A.; An, X.; Shan, S.; Pang, X.; Li, Y.; Fu, X.; Zhang, Y. The microRNAs in the Antennae of Apolygus lucorum (Hemiptera: Miridae): Expression Properties and Targets Prediction. Preprints.org2022, 2022020232. https://doi.org/10.20944/preprints202202.0232.v1.
Khashaveh, A.; An, X.; Shan, S.; Pang, X.; Li, Y.; Fu, X.; Zhang, Y. The microRNAs in the Antennae of Apolygus lucorum (Hemiptera: Miridae): Expression Properties and Targets Prediction. Preprints.org 2022, 2022020232. https://doi.org/10.20944/preprints202202.0232.v1.
Abstract
MicroRNAs (miRNAs) are a class of small non-coding RNAs, which function as regulators of gene expression and contribute in numerous physiological processes. However, little is known referring to miRNAs function in insect chemosensation. In the current study, nine small RNA libraries were constructed and sequenced from the antennae of nymphs, adult males and females of Apolygus lucorum. In total, 399 miRNAs were identified including 275 known and 124 novel miRNAs. Known miRNAs were classified into 71 families, amongst which, 23 families were insect-specific. Expression profile analysis showed that miR-7-5p_1 was the most abundant miRNAs in the antennae of A. lucorum. Altogether, 69708 target genes related to biogenesis, membrane and binding activities were predicted for 399 miRNAs. Particularly, 15 miRNAs were found to target 16 olfactory genes. These miRNAs could be involved in regulation of olfactory-associated genes ex-pression. Comparing the antennae of nymphs, adult males and females, 94 miRNAs were found to be differentially expressed. The expression levels of some differentially expressed miRNAs measured by qPCR were consistent with sequencing results. This study provides a global miRNAs transcriptome in the antennae of A. lucorum and valuable information for further investigation on miRNA-mRNA interactions, especially the functions of miRNAs in regulating chemosensation.
Keywords
Small RNA sequencing; miRNAs; Target prediction; Chemosensory-associated genes; Apolygus lucorum
Subject
Biology and Life Sciences, Insect Science
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
