Misdiagnosis of Bacterial Pathogens by the Diagnostic Centers: A Prospective Route for Antibiotic Resistance

Shanaz Fatema Bristy; Md Reaz Uddin; Bushra Ayat Meghla; Taslima Akter Tisha; Md. Aftab Uddin; Mohammad Tarequl Islam; Masayasu Mie; Eiry Kobatake; Mohib Ullah Khondoker; Md. Ahsanul Haq; Zinia Islam; Sharif Hossain; Nafisa Azmuda; Mohd. Raeed Jamiruddin; Mainul Haque; Nihad Adnan

doi:10.20944/preprints202201.0225.v1

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preprints.org > life sciences > molecular biology > doi: 10.20944/preprints202201.0225.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Misdiagnosis of Bacterial Pathogens by the Diagnostic Centers: A Prospective Route for Antibiotic Resistance

Mohammad Tarequl Islam

Masayasu Mie

Eiry Kobatake

Mohib Ullah Khondoker

Mohd. Raeed Jamiruddin

Mainul Haque

^* ,

Nihad Adnan

Version 1 : Received: 15 January 2022 / Approved: 17 January 2022 / Online: 17 January 2022 (12:35:53 CET)
Version 2 : Received: 18 January 2022 / Approved: 19 January 2022 / Online: 19 January 2022 (11:39:41 CET)

How to cite: Bristy, S.F.; Uddin, M.R.; Meghla, B.A.; Tisha, T.A.; Uddin, M.A.; Islam, M.T.; Mie, M.; Kobatake, E.; Khondoker, M.U.; Haq, M.A.; Islam, Z.; Hossain, S.; Azmuda, N.; Jamiruddin, M.R.; Haque, M.; Adnan, N. Misdiagnosis of Bacterial Pathogens by the Diagnostic Centers: A Prospective Route for Antibiotic Resistance. Preprints 2022, 2022010225. https://doi.org/10.20944/preprints202201.0225.v1. Bristy, S.F.; Uddin, M.R.; Meghla, B.A.; Tisha, T.A.; Uddin, M.A.; Islam, M.T.; Mie, M.; Kobatake, E.; Khondoker, M.U.; Haq, M.A.; Islam, Z.; Hossain, S.; Azmuda, N.; Jamiruddin, M.R.; Haque, M.; Adnan, N. Misdiagnosis of Bacterial Pathogens by the Diagnostic Centers: A Prospective Route for Antibiotic Resistance. Preprints 2022, 2022010225. https://doi.org/10.20944/preprints202201.0225.v1.

Cite as:

Bristy, S.F.; Uddin, M.R.; Meghla, B.A.; Tisha, T.A.; Uddin, M.A.; Islam, M.T.; Mie, M.; Kobatake, E.; Khondoker, M.U.; Haq, M.A.; Islam, Z.; Hossain, S.; Azmuda, N.; Jamiruddin, M.R.; Haque, M.; Adnan, N. Misdiagnosis of Bacterial Pathogens by the Diagnostic Centers: A Prospective Route for Antibiotic Resistance. Preprints 2022, 2022010225. https://doi.org/10.20944/preprints202201.0225.v1. Bristy, S.F.; Uddin, M.R.; Meghla, B.A.; Tisha, T.A.; Uddin, M.A.; Islam, M.T.; Mie, M.; Kobatake, E.; Khondoker, M.U.; Haq, M.A.; Islam, Z.; Hossain, S.; Azmuda, N.; Jamiruddin, M.R.; Haque, M.; Adnan, N. Misdiagnosis of Bacterial Pathogens by the Diagnostic Centers: A Prospective Route for Antibiotic Resistance. Preprints 2022, 2022010225. https://doi.org/10.20944/preprints202201.0225.v1.

Abstract

Successful treatment against infectious agents depends on rapid and accurate detection of the causative organisms. Misdiagnosis can hamper such success while leading to improper advising of antibiotics. In Bangladesh, diagnostic centers detect and identify pathogens through culture and biochemical test-based methods and suggest antibiotics based solely on disk-diffusion methods. In this pilot study, we tried to validate the identity of the isolates characterized by diagnostic facilities near Dhaka. One hundred and twenty pre-characterized clinical isolates were collected and analyzed biochemically and genotypically. Random Amplification of Polymorphic DNA-PCR, rcsA, and phoA genes-based PCR and Loop-Mediated Isothermal Amplification (LAMP)-based identification of Klebsiella pneumoniae and Escherichia coli, respectively, followed by 16S rRNA sequencing confirmed misidentification of some clinical pathogens of other genera as Klebsiella spp. and E. coli. The Clinical and Laboratory Standards Institute (CLSI) provides different guidelines for each group of pathogens, where antibiotic choice, sensitivity pattern, and breakpoint measurement are other. The lack of adherence to proper standards resulting in misdiagnosis may facilitate antibiotic-resistant development. Henceforth, we have observed misidentification of clinical pathogens by the diagnostic centers and suggest that using rapid molecular techniques like LAMP may avoid misdiagnosis and subsequently circumvent antibiotic resistance development.

Keywords

Misdiagnosis; Random Amplification of Polymorphic DNA; 16S rRNA Sequencing; Loop-Mediated Isothermal Amplification; Antibiotic Resistance

Subject

LIFE SCIENCES, Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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