Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Challenges of CRISPR-based Gene Editing in Primary T Cells

Version 1 : Received: 6 January 2022 / Approved: 11 January 2022 / Online: 11 January 2022 (12:57:40 CET)

A peer-reviewed article of this Preprint also exists.

Rezalotfi, A.; Fritz, L.; Förster, R.; Bošnjak, B. Challenges of CRISPR-Based Gene Editing in Primary T Cells. Int. J. Mol. Sci. 2022, 23, 1689. Rezalotfi, A.; Fritz, L.; Förster, R.; Bošnjak, B. Challenges of CRISPR-Based Gene Editing in Primary T Cells. Int. J. Mol. Sci. 2022, 23, 1689.

Journal reference: Int. J. Mol. Sci. 2022, 23, 1689
DOI: 10.3390/ijms23031689

Abstract

Adaptive T cell immunotherapy holds great promise for the successful treatment of leukemia as well as other types of cancers. More recently, it was also shown to be an effective treatment option for chronic virus infections in immunosuppressed patients. Autologous or allogeneic T cells used for immunotherapy are usually genetically modified to express novel T cell or chimeric antigen receptors. The production of such cells was significantly simplified with the CRISPR/Cas system allowing deletion or insertion of novel genes at specific locations within the genome. In this review, we describe recent methodological breakthroughs important for the conduction of these genetic modifications, summarize crucial points to be considered when conducting such experiments, and highlight the potential pitfalls of these approaches.

Keywords

Adoptive T cell therapy; CAR T cells; CRISPR/Cas9; gene modifications; T cells

Subject

LIFE SCIENCES, Immunology

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