Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Direct in vivo Analysis of CBD- and THC-Acid Type Cannabinoids and Classification of Cannabis Cultivars by SpiderMass

Version 1 : Received: 27 December 2021 / Approved: 30 December 2021 / Online: 30 December 2021 (19:02:18 CET)

How to cite: Ogrinc, N.; Schneider, S.; Bourmaud, A.; Salzet, M.; Fournier, I. Direct in vivo Analysis of CBD- and THC-Acid Type Cannabinoids and Classification of Cannabis Cultivars by SpiderMass. Preprints 2021, 2021120493 (doi: 10.20944/preprints202112.0493.v1). Ogrinc, N.; Schneider, S.; Bourmaud, A.; Salzet, M.; Fournier, I. Direct in vivo Analysis of CBD- and THC-Acid Type Cannabinoids and Classification of Cannabis Cultivars by SpiderMass. Preprints 2021, 2021120493 (doi: 10.20944/preprints202112.0493.v1).

Abstract

In the recent years, Cannabis and hemp-based products have become increasingly popular for various applications ranging from recreational use, edibles, beverages to health care products and medicines. The rapid detection and differentiation of phytocannabinoids is, therefore, essential to assess the potency, therapeutic and nutritional values of cannabis cultivars. Here, we implemented the SpiderMass technology for the in vivo detection of cannabidiol acid (CBDA) and tetrahydrocannabinol acid (THCA) and other endogenous organic plant compounds to access distribution gradients within the plants and differentiate cultivars. The SpiderMass system is composed of an IR- laser handheld microsampling probe connected to the mass spectrometer through a transfer tube. The analysis was performed in situ on different plant organs from freshly cultivated Cannabis plants in only a few seconds. SpiderMass analysis easily discriminated the two acid phytocannabinoid isomers by MS/MS and the built statistical models differentiated between four Cannabis cultivars. Different abundancies of acid phytocannabinoids were also found along the plant as well as between different cultivars. All together, these results introduce the direct analysis by SpiderMass as a compelling analytical alternative for forensic and hemp industrial analysis.

Keywords

water-assisted laser desorption/ionization; SpiderMass; cannabinoids; mass spectrometry; plants; in vivo

Subject

CHEMISTRY, Analytical Chemistry

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