Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

MicroRNA-202 Induces Myoblast to Myocyte Differentiation through Targeting Rock-1

Version 1 : Received: 20 December 2021 / Approved: 21 December 2021 / Online: 21 December 2021 (13:57:27 CET)

How to cite: Honardoost, M.; Bourbour, M.; Arefian, E. MicroRNA-202 Induces Myoblast to Myocyte Differentiation through Targeting Rock-1. Preprints 2021, 2021120343 (doi: 10.20944/preprints202112.0343.v1). Honardoost, M.; Bourbour, M.; Arefian, E. MicroRNA-202 Induces Myoblast to Myocyte Differentiation through Targeting Rock-1. Preprints 2021, 2021120343 (doi: 10.20944/preprints202112.0343.v1).

Abstract

Abstract The expression patterns of microRNAs (small non-coding RNAs) are altered in many biological processes such as myogenesis. In this study, we aimed to investigate the impact of predicted miR-202, its target genes Akt2 and Rock-1 as a potential regulator of myoblast in the myocyte differentiation process using the C2C12 cell line. After confirmation of the differentiation process induced by 3% horse serum, the expression level of miRNA and its targets were evaluated. In the following, a luciferase assay was conducted to approve the effect of miRNA on its target. Our results indicated that miR-202 and Akt2 were significantly up-regulated during differentiation, while Rock-1 was downregulated. Co-transfection of miRNA with psiCHECK2-Rock-1 significantly presented that Rock-1 was directly targeted by miR-202. On the contrary, miR-202 has failed to enforce its inhibitory effect on Akt2 expression. In particular, miR-202 seems to be a regulator of muscle differentiation pathway thought targeting Rock-1.

Keywords

miR-202; Rock-1; C2C12; Muscle; Differentiation.

Subject

BIOLOGY, Other

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