Preprint
Review
Visualizing the Complexity of Proteins in Living Cells With Genetic Code Expansion
This version is not peer-reviewed.
Submitted:
20 December 2021
Posted:
21 December 2021
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Abstract
Genetic code expansion has emerged as an enabling tool to provide insight into functions of understudied proteinogenic species such as small proteins and peptides, and to probe protein biophysics in the cellular context. Here we discuss recent technical advances and applications of genetic code expansion in cellular imaging of complex mammalian protein species, along with considerations and challenges upon using the method.
Keywords:
Genetic code expansion
; unnatural amino acid
; fluorescence imaging
Copyright: This open access article is published under a Creative Commons CC BY 4.0 license, which permit the free download, distribution, and reuse, provided that the author and preprint are cited in any reuse.
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