Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Evaluation of B-Cell Kinetics After Acellular Pertussis Vaccination in Four Cohorts of Different Age and Priming Background

Annieck M Diks
ORCID logo ,
Pauline Versteegen
,
Cristina Teodosio
,
Rick J Groenland
,
Bas de Mooij
,
Anne-Marie Buisman
,
Alba Torres-Valle
ORCID logo ,
Martín Pérez-Andrés
,
Alberto Orfao
ORCID logo ,
Guy A.M. Berbers
,
Jacques J.M. van Dongen
* ORCID logo ,
Magdalena A. Berkowska
ORCID logo
Version 1 : Received: 17 November 2021 / Approved: 18 November 2021 / Online: 18 November 2021 (14:18:35 CET)

A peer-reviewed article of this Preprint also exists.

Diks, A.M.; Versteegen, P.; Teodosio, C.; Groenland, R.J.; de Mooij, B.; Buisman, A.-M.; Torres-Valle, A.; Pérez-Andrés, M.; Orfao, A.; Berbers, G.A.M.; van Dongen, J.J.M.; Berkowska, M.A.; on behalf of the IMI-2 PERISCOPE Consortium. Age and Primary Vaccination Background Influence the Plasma Cell Response to Pertussis Booster Vaccination. Vaccines 2022, 10, 136. Diks, A.M.; Versteegen, P.; Teodosio, C.; Groenland, R.J.; de Mooij, B.; Buisman, A.-M.; Torres-Valle, A.; Pérez-Andrés, M.; Orfao, A.; Berbers, G.A.M.; van Dongen, J.J.M.; Berkowska, M.A.; on behalf of the IMI-2 PERISCOPE Consortium. Age and Primary Vaccination Background Influence the Plasma Cell Response to Pertussis Booster Vaccination. Vaccines 2022, 10, 136.

Abstract

Pertussis is a vaccine-preventable disease caused by the bacterium Bordetella pertussis. Over the past years, the incidence and mortality of pertussis increased significantly. A possible cause is the switch from whole cell to acellular pertussis vaccines, although other factors may also contribute. To develop future vaccines and improve current vaccination strategies, it is critical to understand factors influencing the generation of immunological memory. We applied high-dimensional flow cytometry to investigate changes in B cells in individuals of different ages and distinct priming backgrounds upon administration of an acellular pertussis booster vaccine. These findings were correlated to vaccine-specific plasma cells and serum Ig levels. Expansion and maturation of plasma cells 7 days post-vaccination was the most prominent cellular change in all age groups, and was most pronounced for more mature IgG1+ plasma cells. Cellular responses were stronger in individuals primed with whole cell vaccine than in individuals primed with acellular vaccine. Moreover, IgG1+ plasma cell expansion weakly correlated with Prn- and PT- specific serum IgG levels. Our study points at plasma cells as a potential early cellular marker of an immune response and contributes to understanding differences in immune responses between age groups and priming backgrounds.

Keywords

Tdap; flow cytometry; acellular pertussis vaccine; whole cell pertussis vaccine; plasma cells

Subject

Medicine and Pharmacology, Pathology and Pathobiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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