Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Mobile Colistin Resistance Genetic Determinants of Non-typhoid Salmonella enterica Isolates from Russia

Version 1 : Received: 7 November 2021 / Approved: 9 November 2021 / Online: 9 November 2021 (13:46:05 CET)

A peer-reviewed article of this Preprint also exists.

Kuleshov, K.V.; Pavlova, A.S.; Shedko, E.D.; Mikhaylova, Y.V.; Margos, G.; Hepner, S.; Chebotar, I.V.; Korneenko, E.V.; Podkolzin, A.T.; Akimkin, V.G. Mobile Colistin Resistance Genetic Determinants of Non-Typhoid Salmonella enterica Isolates from Russia. Microorganisms 2021, 9, 2515. Kuleshov, K.V.; Pavlova, A.S.; Shedko, E.D.; Mikhaylova, Y.V.; Margos, G.; Hepner, S.; Chebotar, I.V.; Korneenko, E.V.; Podkolzin, A.T.; Akimkin, V.G. Mobile Colistin Resistance Genetic Determinants of Non-Typhoid Salmonella enterica Isolates from Russia. Microorganisms 2021, 9, 2515.

Abstract

Polymyxin resistance, determined by mcr genes located on plasmid DNA, currently pose a high epidemiological threat. Non-typhoid Salmonella (NTS) are one of the key pathogens causing diarrheal diseases. Here, we report the isolation and whole genome sequencing of multidrug colistin-resistant/susceptible isolates of non-typhoid Salmonella enterica serovars carries mcr genes. Non-typhoid strains of Salmonella enterica subsp. enterica were isolated during microbiological monitoring of the environment, food, and diarrheal disease patients between 2018 and 2020 in Russia (n=586). mcr-1 genes were detected using a previously developed qPCR assay and whole genome sequencing of mcr positive isolates was performed by both short-read (Illumina) and long-read (Oxford Nanopore) approaches. Three colistin-resistant isolates including two isolates of S. Enteritidis and one isolate of S. Bovismorbificans carried the mcr-1.1 gene located on IncX4 and IncI2 conjugative plasmids, respectively. The phenotypically colistin-susceptible isolate of S. Typhimurium carried a mcr-9 gene on plasmid IncHI2. In conclusion, we present the first three cases of mcr gene carrying NTS isolates detected in Russia with both outbreak and sporadic epidemiological background.

Keywords

whole genome sequencing; antibiotic resistance; Salmonella Enteritidis; Salmonella Typhimurium; Salmonella Bovismorbificans; colistin resistance; mcr-1; mcr-9

Subject

Biology and Life Sciences, Immunology and Microbiology

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