Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Determination of Avermectins Residues in Soybean, Bean and Maize Using a QuEChERS-based Method and Ultra-high-performance Liquid Chromatography Coupled to Tandem Mass Spectrometry

Version 1 : Received: 24 October 2021 / Approved: 25 October 2021 / Online: 25 October 2021 (15:50:47 CEST)

A peer-reviewed article of this Preprint also exists.

Uczay, F.; Bandeira, N.M.G.; Floriano, L.; Prestes, O.D.; Adaime, M.B.; Zanella, R. Determination of Avermectins Residues in Soybean, Bean, and Maize Using a QuEChERS-Based Method and Ultra-High-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry. Separations 2021, 8, 214. Uczay, F.; Bandeira, N.M.G.; Floriano, L.; Prestes, O.D.; Adaime, M.B.; Zanella, R. Determination of Avermectins Residues in Soybean, Bean, and Maize Using a QuEChERS-Based Method and Ultra-High-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry. Separations 2021, 8, 214.

Journal reference: Separations 2021, 8, 214
DOI: 10.3390/separations8110214

Abstract

Soybean, maize and bean are crops of great economic importance, but in the last years suffered with infestations of the caterpillar Helicoverpa armigera, being the main problem the resistance of this pest to most pesticides. Avermectin emamectin benzoate was recently released to control this pest. Other avermectins, like abamectin, doramectin, eprinomectin and ivermectin are used in large scale because they potent acaricidal, anthelmintic, and insecticidal activities. Thus, a simple and fast method for the determination of avermectins in these crops based on a quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction procedure and ultra-high performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) analysis was developed and validated. For extraction, water followed by acetonitrile:isopropanol and a partition step with salts was stablished. With the clean-up step using activated EMR-Lipid, limits of detection of 1.2 μg kg-1 for abamectin, doramectin, emamectin benzoate and ivermectin, and of 2.4 μg kg-1 for eprinomectin were achieved. Accuracy and precision evaluated at low levels presented satisfactory results. The method was successfully applied in commercial samples and is a good alternative for routine analysis.

Keywords

Macrocyclic lactones; agricultural crops; food; sample preparation; UHPLC-MS/MS

Subject

CHEMISTRY, Analytical Chemistry

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