Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation

Version 1 : Received: 13 October 2021 / Approved: 14 October 2021 / Online: 14 October 2021 (10:23:28 CEST)

How to cite: Kaddour, H.; Tranquille, M.; Okeoma, C.M. The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation. Preprints 2021, 2021100211 (doi: 10.20944/preprints202110.0211.v1). Kaddour, H.; Tranquille, M.; Okeoma, C.M. The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation. Preprints 2021, 2021100211 (doi: 10.20944/preprints202110.0211.v1).

Abstract

Extracellular vesicles (EVs) are cell-derived membranous particles secreted by all cell types into the extracellular milieu. EVs carry, protect, and transport a wide array of bioactive cargoes to recipient/target cells. EVs regulate physiological and pathophysiological processes in recipient cells and are important in therapeutics/drug delivery. Despite these great attributes of EVs, an efficient protocol for EV separation from biofluids is lacking. Numerous techniques have been adapted for the separation of EVs with size exclusion chromatography (SEC)-based methods being the most promising. Here, we review the SEC protocols used for EV separation, and discuss opportunities for significant improvements, such as the development of novel particle purification liquid chromatography (PPLC) system capable of tandem purification and characterization of biological and synthetic particles with near-single vesicle resolution. Finally, we identify future perspectives and current issues to make PPLC a tool capable of providing a unified, automated, adaptable, yet simple and affordable particle separation resource.

Keywords

Extracellular Vesicles (EVs); Exosomes; Size Exclusion Chromatography (SEC); gradient Size Exclusion Chromatography (gSEC); Ion Exchange Chromatography; Hybrid Chromatography

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