Xu, Y.; Harvey, P.J. Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga Dunaliella salina. Mar. Drugs2021, 19, 595.
Xu, Y.; Harvey, P.J. Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga Dunaliella salina. Mar. Drugs 2021, 19, 595.
Xu, Y.; Harvey, P.J. Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga Dunaliella salina. Mar. Drugs2021, 19, 595.
Xu, Y.; Harvey, P.J. Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga Dunaliella salina. Mar. Drugs 2021, 19, 595.
Abstract
Phytoene is a colourless natural carotenoid that absorbs UV light and provides antioxidant and anti-inflammatory activities as well as protection against photodamage. It is therefore valued for its skin health and aesthetic benefits by the cosmetic industry, as well as by the health food sector. The halotolerant green microalga Dunaliella salina is one of the richest sources of natural carotenoids. We have previously investigated over-production of phytoene in D. salina after cultivation with the well-characterised mitosis inhibitor, chlorpropham. In this study, 15 herbicides with different modes of action were tested for their potential to promote phytoene accumulation. All herbicides showed different levels of capabilities to support phytoene over-production in D. salina. Most significantly, the two mitosis inhibitors tested in this study, propyzamide and chlorpropham, showed similar capacities to support over-production of phytoene by D. salina cultures, as phytoene desaturase inhibitors. The cellular content of phytoene increased by over 10-fold within 48 h after treatment with the mitosis inhibitors compared to untreated cultures. Results indicate a general effect of mitosis inhibitors on phytoene accumulation in D. salina. Furthermore, red light was found to significantly enhance the phytoene yield when used in combination with effective inhibitor treatments. Red light can be applied to maximize the production of phytoene from D. salina.
Keywords
Phytoene, Dunaliella salina, mitosis inhibitors, herbicides, red light
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
