Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Microfluidic Lab-On-a-Chip based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells

Elisa Lambert
* ,
Rémi Manczak
,
Elodie Barthout
,
Sofiane Saada
,
Elena Porcù
,
Francesca Maule
,
Barbara BESSETTE
ORCID logo ,
Giampietro Viola
ORCID logo ,
Luca Persano
,
Claire DALMAY
,
Fabrice Lalloué
ORCID logo ,
Arnaud POTHIER
*
Version 1 : Received: 20 September 2021 / Approved: 24 September 2021 / Online: 24 September 2021 (11:11:35 CEST)

How to cite: Lambert, E.; Manczak, R.; Barthout, E.; Saada, S.; Porcù, E.; Maule, F.; BESSETTE, B.; Viola, G.; Persano, L.; DALMAY, C.; Lalloué, F.; POTHIER, A. Microfluidic Lab-On-a-Chip based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells. Preprints 2021, 2021090424. https://doi.org/10.20944/preprints202109.0424.v1 Lambert, E.; Manczak, R.; Barthout, E.; Saada, S.; Porcù, E.; Maule, F.; BESSETTE, B.; Viola, G.; Persano, L.; DALMAY, C.; Lalloué, F.; POTHIER, A. Microfluidic Lab-On-a-Chip based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells. Preprints 2021, 2021090424. https://doi.org/10.20944/preprints202109.0424.v1

Abstract

Glioblastoma (GBM) is one of the most aggressive solid tumors, particularly due to the presence of cancer stem cells (CSCs). Today the characterization of this type of cells with an efficient, fast and low-cost method remains an issue. Hence, we have developed a microfluidic lab-on-a-chip based on dielectrophoresis (DEP) single cell electro-manipulation to measure the two crossover frequencies: fx01 in low frequency range (below 500 kHz) and fx02 in Ultra High Frequency range (UHF, above 50 MHz). First, in vitro conditions were investigated. U87-MG cell lines were cultured in different conditions in order to induce an undifferentiated phenotype. Then, ex vivo GBM cells from patients’ primary cell culture, were passed through the developed microfluidic system and characterized in order to reflect clinical conditions. This article demonstrates that the usual exploitation of low frequency range DEP does not allow the discrimination of the undifferentiated from the differentiated phenotypes of GBM cells. However, the presented study highlights the use of UHF-DEP as a relevant discriminant parameter. The proposed microfluidic lab-on-a-chip is able to follow the kinetic of U87-MG phenotype transformation in a CSC enrichment medium and their cancer stem cells phenotype acquirement.

Keywords

high frequency dielectrophoresis; glioblastoma cells; single cell manipulation; microfluidic point-of-care device; cancer stem cells

Subject

Medicine and Pharmacology, Oncology and Oncogenics

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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