Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

A New Double-Stranded RNA Mycovirus in Cryphonectria naterciae is Able to Cross the Species Barrier and is Deleterious to a New Host

Version 1 : Received: 16 September 2021 / Approved: 20 September 2021 / Online: 20 September 2021 (10:52:30 CEST)

A peer-reviewed article of this Preprint also exists.

Cornejo, C.; Hisano, S.; Bragança, H.; Suzuki, N.; Rigling, D. A New Double-Stranded RNA Mycovirus in Cryphonectria naterciae Is Able to Cross the Species Barrier and Is Deleterious to a New Host. J. Fungi 2021, 7, 861. Cornejo, C.; Hisano, S.; Bragança, H.; Suzuki, N.; Rigling, D. A New Double-Stranded RNA Mycovirus in Cryphonectria naterciae Is Able to Cross the Species Barrier and Is Deleterious to a New Host. J. Fungi 2021, 7, 861.

Journal reference: J. Fungi 2021, 7, 861
DOI: 10.3390/jof7100861

Abstract

Cryphonectria is a fungal genus associated with economically significant disease of trees. Herein we characterized a novel double-stranded RNA virus from the fungal species Cryphonectria naterciae, a species unexplored as a virus host. De novo assembly of RNA-seq data and Sanger sequencing of RACE (rapid amplification of cDNA ends) clones gave the complete, non-segmented genome (10,164 bp) of the virus termed Cryphonectria naterciae fusagravirus (CnFGV1) that was phylogenetically placed within the previously proposed viral family Fusagraviridae. Of 31 field-collected strains of C. naterciae, 40% tested CnFGV1-positive. Co-cultivation resulted in within-species transmission of CnFGV1 to virus-free strains of C. naterciae. Comparison of the mycelium phenotype and the growth rate of CnFGV1-infected and virus-free isogenic strains revealed frequent sectoring and growth reduction in C. naterciae. Co-culturing also led to cross-species transmission of CnFGV1 to Cryphonectria carpinicola and Cryphonectria radicalis, but not to Cryphonectria parasitica. The virus-infected C. naterciae and the experimentally infected Cryphonectria spp. readily transmitted CnFGV1 through asexual spores to the next generation. CnFGV1 strongly reduced conidiation and in some cases vegetative growth of C. carpinicola, which is involved in the European hornbeam disease. This study is the first report of a fusagravirus in the family Cryphonectriaceae and lays the groundwork for assessing a hypovirulence effect of CnFGV1 against the hornbeam decline in Europe.

Keywords

Virus diversity; Quercus suber; Castanea sativa; hornbeam decline; hypovirulence; bio-control; Portugal.

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.