Version 1
: Received: 12 September 2021 / Approved: 15 September 2021 / Online: 15 September 2021 (13:14:49 CEST)
How to cite:
Ximenes Rodrigues, A.M.; Gomes dos Santos, B.N.; Machado, R.S.; de Sousa Carmo, R.R.; de Oliveira, M.P.; Lopes Citó, A.M.D.G.; de Medeiros Carvalho, M.D.G.F.; dos Santos Leite, P.T. Essential Oil of Lippia origanoides H.B.K.: A Promising Compound Acetylcholinesterase Inhibitors for the Treatment of Alzheimer’s Disease. Preprints2021, 2021090261. https://doi.org/10.20944/preprints202109.0261.v1
Ximenes Rodrigues, A.M.; Gomes dos Santos, B.N.; Machado, R.S.; de Sousa Carmo, R.R.; de Oliveira, M.P.; Lopes Citó, A.M.D.G.; de Medeiros Carvalho, M.D.G.F.; dos Santos Leite, P.T. Essential Oil of Lippia origanoides H.B.K.: A Promising Compound Acetylcholinesterase Inhibitors for the Treatment of Alzheimer’s Disease. Preprints 2021, 2021090261. https://doi.org/10.20944/preprints202109.0261.v1
Ximenes Rodrigues, A.M.; Gomes dos Santos, B.N.; Machado, R.S.; de Sousa Carmo, R.R.; de Oliveira, M.P.; Lopes Citó, A.M.D.G.; de Medeiros Carvalho, M.D.G.F.; dos Santos Leite, P.T. Essential Oil of Lippia origanoides H.B.K.: A Promising Compound Acetylcholinesterase Inhibitors for the Treatment of Alzheimer’s Disease. Preprints2021, 2021090261. https://doi.org/10.20944/preprints202109.0261.v1
APA Style
Ximenes Rodrigues, A.M., Gomes dos Santos, B.N., Machado, R.S., de Sousa Carmo, R.R., de Oliveira, M.P., Lopes Citó, A.M.D.G., de Medeiros Carvalho, M.D.G.F., & dos Santos Leite, P.T. (2021). Essential Oil of <em>Lippia origanoides</em> H.B.K.: A Promising Compound Acetylcholinesterase Inhibitors for the Treatment of Alzheimer’s Disease. Preprints. https://doi.org/10.20944/preprints202109.0261.v1
Chicago/Turabian Style
Ximenes Rodrigues, A.M., Maria das Graças Freire de Medeiros Carvalho and Paulo Tércio dos Santos Leite. 2021 "Essential Oil of <em>Lippia origanoides</em> H.B.K.: A Promising Compound Acetylcholinesterase Inhibitors for the Treatment of Alzheimer’s Disease" Preprints. https://doi.org/10.20944/preprints202109.0261.v1
Abstract
Alzheimer's disease is characterized by a progressive decline of cognitive functions. The class of drugs used for the treatment are acetylcholinesterase inhibitors. Essential oils have contributed to folk medicine and discovery of new drugs for a long time. The purpose of the study was to investigate the in vitro and in silico the anti-acetylcholinesterase activity, as well as acute toxicity of the essential oil of Lippia origanoides. EOLO was obtained by hydrostelting and analyzed by gas chromatography-mass spectrometry. The inhibition assay of acetylcholinesterase enzyme activity was evaluated in vitro, as well as in silico by docking. The effects of EOLO on hematological, biochemical and behavioral parameters were analyzed in mices. We expose that EOLO shows good anti-acetylcholinesterase activity and low toxicity, possibly resulting from the action of the majority compounds thymol, carvacrol and p-cymene. The anti-acetylcholinesterase potential in vitro demonstrating a 70% inhibition. The docking results elucidated the participation of the major phenolics in AChE inhibition by interacting with the catalytic cavity of AchE. The acute oral toxicity test classified as low toxicity. These results contribute to expand the knowledge about essential oil of Lippia origanoides. Therefore, appears to be promising for herbal medicine production with anti-acetylcholinesterase and antioxidant activity.
Keywords
Acetylcholinesterase; Dementia; Essencial oil
Subject
Medicine and Pharmacology, Pharmacology and Toxicology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
