Putri, A.; Nurtjahjaningsih, I.; Prihatini, I.; Nai'em, M.; Indrioko, S.; Rahayu, S. Callus Induction from Root Fragments of Falcataria moluccana Plantlets. Preprints2021, 2021090227. https://doi.org/10.20944/preprints202109.0227.v1
APA Style
Putri, A., Nurtjahjaningsih, I., Prihatini, I., Nai'em, M., Indrioko, S., & Rahayu, S. (2021). Callus Induction from Root Fragments of Falcataria moluccana Plantlets. Preprints. https://doi.org/10.20944/preprints202109.0227.v1
Chicago/Turabian Style
Putri, A., Sapto Indrioko and Sri Rahayu. 2021 "Callus Induction from Root Fragments of Falcataria moluccana Plantlets" Preprints. https://doi.org/10.20944/preprints202109.0227.v1
Abstract
Callus induction of F. moluccana (sengon) was still an obstacle to indirect organogenesis regeneration. The purpose of the study was to determine the callus induction formation from root fragments of F. moluccana plantlets. Primary explants (fragments of roots) were cultivated on MS induction basal media and three concentration combination of PGRs (BAP and NAA): 0.5 ml/l BAP; 0,1 ml/l NAA (single PGR); and combination of 0.5 ml/l BAP + 0.1 ml/l NAA (double PGR). When roots were used as explants, high formation rates of callus (more than 70%) were obtained. Highest formation rates of callus were in NAA added at all clones (12 clones), then BAP added (7 clones) and BAP + NAA added (5 clones). The results indicated that BAP and NAA concentrations used in the media were influence the producing callus and affect the amount of callus produced from roots of sengon. The addition of NAA also gives higher callus proliferation results than the addition of BAP or the addition of a combination of the two hormones. The results indicated that BAP and NAA concentrations used in the media were influence the producing callus and affect the amount of callus produced from roots of sengon.
Keywords
PGRs; callus induction; indirect organogenesis
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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