Version 1
: Received: 7 September 2021 / Approved: 8 September 2021 / Online: 8 September 2021 (10:50:49 CEST)
How to cite:
Limoli, C.; Limoli, P.G.; Nebbioso, M. Phenotype Characterization of Human Adipose-Derived Stem Cell by Flow Cytometry. Preprints2021, 2021090139. https://doi.org/10.20944/preprints202109.0139.v1
Limoli, C.; Limoli, P.G.; Nebbioso, M. Phenotype Characterization of Human Adipose-Derived Stem Cell by Flow Cytometry. Preprints 2021, 2021090139. https://doi.org/10.20944/preprints202109.0139.v1
Limoli, C.; Limoli, P.G.; Nebbioso, M. Phenotype Characterization of Human Adipose-Derived Stem Cell by Flow Cytometry. Preprints2021, 2021090139. https://doi.org/10.20944/preprints202109.0139.v1
APA Style
Limoli, C., Limoli, P.G., & Nebbioso, M. (2021). Phenotype Characterization of Human Adipose-Derived Stem Cell by Flow Cytometry. Preprints. https://doi.org/10.20944/preprints202109.0139.v1
Chicago/Turabian Style
Limoli, C., Paolo Giuseppe Limoli and Marcella Nebbioso. 2021 "Phenotype Characterization of Human Adipose-Derived Stem Cell by Flow Cytometry" Preprints. https://doi.org/10.20944/preprints202109.0139.v1
Abstract
Background: Developing an efficient and standardized method to isolate and characterize adipose-derived stem cells (ASCs) from the stromal vascular fraction (SVF) of the adipose tissue for clinical application represents one of the major challenges in cell therapy and tissue engineering. Methods: In this study, we proposed an innovative, non-enzymatic protocol to collect clinically useful ASCs within freshly isolated SVF from adipose tissue by centrifugation of the infranatant portion of lipoaspirate and to determine the characteristic cytofluorimetric pattern, prior to in vitro culture. Results: The SVF yielded a mean of 73,32 \pm\ 10,89% cell viability evaluated with CALCEINA-FITC, i.e. cell-permeant dye. The ASCs were positive for PC7-labeled mAb anti-CD34 and negative for both PE-labeled mAb anti-CD31 and APC-labeled mAb anti-CD45. The frequency of ASCs estimated according to the panel of cell surface markers used was 51,06%\ \pm 5,26% versus the unstained ASCs subpopulation that was 0,74%\pm0,84% (P<0.0001). The ASCs events/\muL were 1602,13/\muL \pm 731,87/\muL. Conclusion: Our findings suggested that ASCs found in freshly isolated adipose SVF obtained by centrifugation of lipoaspirate can be immunophenotypically identified with a basic panel of cell surface markers. These findings aimed to provide standardization and contribute to reducing the inconsistency on reported cell surface antigens of ASC derived from the existing literature.
Biology and Life Sciences, Cell and Developmental Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
