Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

CAST as a Potential Oncogene From Machine Searching in Gastric Cancer Infiltrated with Macrophage and Associated with Lgr5

Version 1 : Received: 18 August 2021 / Approved: 20 August 2021 / Online: 20 August 2021 (14:00:27 CEST)

How to cite: Yang, K.; Li, C.; Chang, R.; Wang, J.; Tarng, Y.; Chen, J. CAST as a Potential Oncogene From Machine Searching in Gastric Cancer Infiltrated with Macrophage and Associated with Lgr5. Preprints 2021, 2021080418 (doi: 10.20944/preprints202108.0418.v1). Yang, K.; Li, C.; Chang, R.; Wang, J.; Tarng, Y.; Chen, J. CAST as a Potential Oncogene From Machine Searching in Gastric Cancer Infiltrated with Macrophage and Associated with Lgr5. Preprints 2021, 2021080418 (doi: 10.20944/preprints202108.0418.v1).

Abstract

Background: Gastric cancer (GC) is one of the leading malignancy diseases worldwide, especially in Asian. CAST is a potential oncogene in GC carcinogenesis process. The character of macrophage infiltration in GC microenvironment was also unaddressed. Methods: We first applied machine searching in gene candidate evaluation of GC. CAST expression was analyzed via the Human Protein Atlas (HPA) and Gene Expression Profiling Interactive Analysis 2 (GEPIA2) database. Protein-protein interaction (PPI) network was downloaded from STRING. We investigated the impact of CAST on clinical prognosis using Kaplan-Meier plotter. The correlations between CAST and Lgr5 and macrophage infiltration in GC was surveyed via TIMER 2.0. Finally, GeneMANIA was also used to evaluate the possible functional linkage between genes. Results: After machine-assisted searching, CAST expression was found signicant difference in the overall survival of GC patients. STRING revealed CAST related proteomics and transcriptomics associations, mainly about CAPN family. Moreover, CAST significantly impacts the prognosis of GC from other datasets validation. Notably, high CAST expression was correlated with worse overall survival in GC patients (hazard ratio = 1.59; logrank P = 9.4 x 10-8). CAST and Lgr5 expressions were both positively correlated with WNT 2 and WNT 2B. Among GC patients in several datasets, CAST and macrophage infiltration evaluated together showed no obvious trend toward poor clinical overall survival. Conclusion: CAST plays an important role in GC clinical prognosis and is associated with WNT 2/WNT 2B/Lgr5. Our study denmostrated that CAST in GC overall survival is regulated by macrophage infiltration.

Keywords

CAST; Lgr5; WNT; Gastric cancer; Machine assisted searching; macrophage

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.