Chikungunya (Togaviridae) and Dengue 2 (Flaviviridae) Viruses Isolated from Aedes aegypti Mosquitoes by qRT-PCR Technique: Xenosurveillance for Arboviruses Circulating in Burkina Faso

Aristide Sawdetuo Hien; Ibrahim Sangaré; Eric L. Parfait Ouattara; Simon  P. Sawadogo; Diloma D. Soma; Maiga Hamidou; Abdoulaye Diabaté; Emmanuel Bonnet; Valéry Ridde; Florence Fournet; Carina Kaupra; Jérémy Bouyer; Adly M.M. Abd-Alla; Frances M. Hawkes; Roch K. Dabiré

doi:10.20944/preprints202108.0077.v1

How to cite: Hien, A.S.; Sangaré, I.; Ouattara, E.L.P.; Sawadogo, S..P.; Soma, D.D.; Hamidou, M.; Diabaté, A.; Bonnet, E.; Ridde, V.; Fournet, F.; Kaupra, C.; Bouyer, J.; Abd-Alla, A.M.; Hawkes, F.M.; Dabiré, R.K. Chikungunya (Togaviridae) and Dengue 2 (Flaviviridae) Viruses Isolated from Aedes aegypti Mosquitoes by qRT-PCR Technique: Xenosurveillance for Arboviruses Circulating in Burkina Faso. Preprints 2021, 2021080077. https://doi.org/10.20944/preprints202108.0077.v1 Hien, A.S.; Sangaré, I.; Ouattara, E.L.P.; Sawadogo, S..P.; Soma, D.D.; Hamidou, M.; Diabaté, A.; Bonnet, E.; Ridde, V.; Fournet, F.; Kaupra, C.; Bouyer, J.; Abd-Alla, A.M.; Hawkes, F.M.; Dabiré, R.K. Chikungunya (Togaviridae) and Dengue 2 (Flaviviridae) Viruses Isolated from Aedes aegypti Mosquitoes by qRT-PCR Technique: Xenosurveillance for Arboviruses Circulating in Burkina Faso. Preprints 2021, 2021080077. https://doi.org/10.20944/preprints202108.0077.v1

Abstract

Abstract: In 2016, we conducted an entomological survey in a railway transect between Banfora and Ouagadougou, Burkina Faso. The aim was to evaluate the risk factors for arbovirus epidemics, including vector infection status, in areas representative of the country. Aedes aegypti mosquitoes were collected at larval stage from four study sites and reared until adult stage and kept in RNAlater for detection of arbovirus RNA. In the laboratory, the mosquito specimens were screened for dengue virus (DENV) and chikungunya virus (CHIKV) using one step real-time qRT-PCR. We detected one DENV-2 positive pool from Ouagadougou, giving a minimum infec-tion rate (MIR) of 16.67, and 6 CHIKV positive pools, giving a MIR of 66.67 from Ouagadougou, but also in Banfora and Boromo. The qRT-PCR is a useful tool for the surveillance of arboviruses of public health importance in Burkina Faso and may be incorporated into disease surveillance and control programs in Burkina Faso.

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Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Chikungunya (Togaviridae) and Dengue 2 (Flaviviridae) Viruses Isolated from Aedes aegypti Mosquitoes by qRT-PCR Technique: Xenosurveillance for Arboviruses Circulating in Burkina Faso

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