Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Chemical Characterization of Atlantic Cod (Gadus morhua) Collagen Hydrolyzed Using Enzyme Preparation Derived from Red King Crab (Paralithodes camtschaticus) and Its Potential as a Core Component of Bacterial Culture Medium

Vitaliy Yu. Novikov
ORCID logo ,
Nadezhda V. Shumskaya
,
Vyacheslav A. Mukhin
,
Konstantin V. Zolotarev
* ORCID logo ,
Anton N. Mikhailov
,
Valeriya I. Nakhod
,
Marina V. Mikhailova
Version 1 : Received: 28 July 2021 / Approved: 29 July 2021 / Online: 29 July 2021 (10:25:50 CEST)

A peer-reviewed article of this Preprint also exists.

Novikov, V.Y.; Shumskaya, N.V.; Mukhin, V.A.; Zolotarev, K.V.; Mikhailov, A.N.; Nakhod, V.I.; Mikhailova, M.V. Chemical Characterization of Atlantic Cod (Gadusmorhua) Collagen Hydrolyzed Using Enzyme Preparation Derived from Red King Crab (Paralithodes Camtschaticus) and Its Potential as a Core Component of Bacterial Culture Medium. Mar. Drugs 2021, 19, 472. Novikov, V.Y.; Shumskaya, N.V.; Mukhin, V.A.; Zolotarev, K.V.; Mikhailov, A.N.; Nakhod, V.I.; Mikhailova, M.V. Chemical Characterization of Atlantic Cod (Gadusmorhua) Collagen Hydrolyzed Using Enzyme Preparation Derived from Red King Crab (Paralithodes Camtschaticus) and Its Potential as a Core Component of Bacterial Culture Medium. Mar. Drugs 2021, 19, 472.

Abstract

The Atlantic cod (Gadus morhua) and red king crab (Paralithodes camtschaticus) processing wastes are massive and unutilized in the Murmansk region of Russia. The samples of skin-containing waste of Atlantic cod fillets production were hydrolyzed using enzyme preparations derived from red king crab hepatopancreases, porcine pancreases and Bacillus subtilis bacteria. The activity of enzymes from crab hepatopancreases was significantly higher than the activity of enzymes derived from other sources. The optimal conditions of the hydrolysis process have been figured out. The samples of cod processing waste hydrolysate were analyzed for amino acid composition and molecular weight distribution. The samples of hydrolysate were used as core components for bacterial culture medium samples. The efficiency of the medium samples was tested for Escherichia coli growth rate; the most efficient sample had efficiency 95.3% of that of a commercially available medium based on fish meal. Substitution of medium components with those derived from industrial by-products is one of the ways to decrease a cost of a culture medium in biopharmaceutical drug production.

Keywords

processing waste; Atlantic cod; collagen; red king crab; enzyme preparation; collagen hydrolysate; culture medium

Subject

Engineering, Automotive Engineering

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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