Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

A Portable Device for LAMP Based Detection of Sars-Cov-2

Version 1 : Received: 27 July 2021 / Approved: 28 July 2021 / Online: 28 July 2021 (17:49:56 CEST)

How to cite: Sreejith, K.R.; Umer, M.; Soda, N.; Kasetsirikul, S.; Shiddiky, M.; Nguyen, N. A Portable Device for LAMP Based Detection of Sars-Cov-2. Preprints 2021, 2021070640 (doi: 10.20944/preprints202107.0640.v1). Sreejith, K.R.; Umer, M.; Soda, N.; Kasetsirikul, S.; Shiddiky, M.; Nguyen, N. A Portable Device for LAMP Based Detection of Sars-Cov-2. Preprints 2021, 2021070640 (doi: 10.20944/preprints202107.0640.v1).

Abstract

This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for the rapid detection of SARS-CoV-2. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 minutes using clinically relevant starting concentrations of the synthetic template. Time-to-signal data for this device are considerably lower compared to standard qPCR machine (~10-20 minutes vs >38 minutes) for 1×105 starting template copy number. The device in its fully optimized and characterized state can potentially be used as simple to operate, rapid, sensitive, and inexpensive platform for population screening as well as point-of-need SARS-CoV-2 detection and patient management.

Keywords

SARS-COV-2; Loop-mediated isothermal amplification; Portable device.

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.