Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

A Portable Device for LAMP Based Detection of Sars-Cov-2

Kamalalayam Rajan Sreejith
* ,
Muhammad Umer
ORCID logo ,
Narshone Soda
ORCID logo ,
Surasak Kasetsirikul
,
Muhammad Shiddiky
,
Nam-Trung Nguyen
* ORCID logo
Version 1 : Received: 27 July 2021 / Approved: 28 July 2021 / Online: 28 July 2021 (17:49:56 CEST)

A peer-reviewed article of this Preprint also exists.

Sreejith, K.R.; Umer, M.; Dirr, L.; Bailly, B.; Guillon, P.; von Itzstein, M.; Soda, N.; Kasetsirikul, S.; Shiddiky, M.J.A.; Nguyen, N.-T. A Portable Device for LAMP Based Detection of SARS-CoV-2. Micromachines 2021, 12, 1151. Sreejith, K.R.; Umer, M.; Dirr, L.; Bailly, B.; Guillon, P.; von Itzstein, M.; Soda, N.; Kasetsirikul, S.; Shiddiky, M.J.A.; Nguyen, N.-T. A Portable Device for LAMP Based Detection of SARS-CoV-2. Micromachines 2021, 12, 1151.

Abstract

This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for the rapid detection of SARS-CoV-2. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 minutes using clinically relevant starting concentrations of the synthetic template. Time-to-signal data for this device are considerably lower compared to standard qPCR machine (~10-20 minutes vs >38 minutes) for 1×105 starting template copy number. The device in its fully optimized and characterized state can potentially be used as simple to operate, rapid, sensitive, and inexpensive platform for population screening as well as point-of-need SARS-CoV-2 detection and patient management.

Keywords

SARS-COV-2; Loop-mediated isothermal amplification; Portable device.

Subject

Engineering, Automotive Engineering

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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