Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

MalaSelect: a Selective Culture Medium for Malassezia Species Isolation

Version 1 : Received: 26 July 2021 / Approved: 27 July 2021 / Online: 27 July 2021 (11:29:17 CEST)

A peer-reviewed article of this Preprint also exists.

Abdillah, A.; Ranque, S. MalaSelect: A Selective Culture Medium for Malassezia Species. J. Fungi 2021, 7, 824. Abdillah, A.; Ranque, S. MalaSelect: A Selective Culture Medium for Malassezia Species. J. Fungi 2021, 7, 824.


Malassezia species are fastidious and slow-growing yeasts whose isolation from polymicrobial samples is hampered by fast-growing microorganisms. Malassezia selective culture media are needed because Malassezia are resistant to cycloheximide, but some fungi, including the chief human commensal Candida albicans resist to this compound. This study aimed to test whether the macrolide rapamycin could be used in combination with cycloheximide to develop a Malassezia-selective culture medium. Rapamycin susceptibility testing was performed via microdilution assays in modified Dixon against M. furfur and five Candida spp. The MIC was the lowest concentration producing 90% growth inhibition. Rapamycin medium ± cycloheximide 500 mg/L was also added to FastFung solid and yeast suspensions were inoculated and incubated for 72h. Rapamycin MICs against Candida spp. ranged from 0.5 to 2 mg/L, except for C. krusei whose MIC was >32 mg/L. M. furfur stains were rapamycin resistant. Rapamycin and cycloheximide supplementation of the FastFung medium effectively inhibited the growth of non-Malassezia yeast, including the cycloheximide-resistant C. albicans and C. tropicalis. Based on our findings, we recommend using this “MalaSelect” medium for Malassezia isolation and culture from polymicrobial samples.


Malassezia; selective culture medium; Rapamycin; isolation; polymicrobial samples.


Biology and Life Sciences, Biochemistry and Molecular Biology

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