Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Occurrence of Shiga Toxin-Producing Escherichia coli Carrying Antimicrobial Resistance Genes in Sheep on Smallholdings in Bangladesh

Version 1 : Received: 17 July 2021 / Approved: 19 July 2021 / Online: 19 July 2021 (11:12:01 CEST)

How to cite: Gupta, M.D.; Sen, A.; Shaha, M.; Dutta, A.; Das, A. Occurrence of Shiga Toxin-Producing Escherichia coli Carrying Antimicrobial Resistance Genes in Sheep on Smallholdings in Bangladesh. Preprints 2021, 2021070405 (doi: 10.20944/preprints202107.0405.v1). Gupta, M.D.; Sen, A.; Shaha, M.; Dutta, A.; Das, A. Occurrence of Shiga Toxin-Producing Escherichia coli Carrying Antimicrobial Resistance Genes in Sheep on Smallholdings in Bangladesh. Preprints 2021, 2021070405 (doi: 10.20944/preprints202107.0405.v1).

Abstract

Inappropriate antimicrobial treatment can pose a risk for developing resistance against antimi-crobial drugs in bacteria. Close human contact might have a higher chance of being transmitted to humans from sheep if the sheep population is a potential reservoir of zoonotic pathogens such as shiga toxin-producing Escherichia coli (E. coli) (STEC). Therefore, this study aimed to exam-ine the sheep population in rural Bangladesh for antimicrobial resistant STEC. We screened 200 faecal samples collected from sheep in three Upazila from the Chattogram district. Phenotypical-ly positive E. coli isolates were examined for two shiga toxin-producing genes – stx1 and stx2. PCR positive STEC isolates were investigated for the presence of antimicrobial resistance genes- blaTEM, sul1 and sul2. In total, 123 of the 200 tested samples were confirmed positive E. coli by cul-tured based methods. PCR results show 17(13.8%) E. coli isolates harboured ≥ one virulent gene (stx1 or/and stx2) of STEC. Six of the tested STEC isolates exhibited blaTEM gene; eight STEC isolates had sul1 gene, and sul2 gene was detected in ten STEC isolates. To our knowledge, this study is the first to reveal a significant proportion of STEC isolated from sheep in rural Bangla-desh harbouring antimicrobial resistance genes.

Subject Areas

Sheep; E. coli; shiga-toxin; antimicrobial resistance genes

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