Review
Version 1
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Quantitative Approaches to Study Retinal Neurogenesis
Version 1
: Received: 5 July 2021 / Approved: 13 July 2021 / Online: 13 July 2021 (12:18:20 CEST)
A peer-reviewed article of this Preprint also exists.
Pérez-Dones, D.; Ledesma-Terrón, M.; Míguez, D.G. Quantitative Approaches to Study Retinal Neurogenesis. Biomedicines 2021, 9, 1222. Pérez-Dones, D.; Ledesma-Terrón, M.; Míguez, D.G. Quantitative Approaches to Study Retinal Neurogenesis. Biomedicines 2021, 9, 1222.
Abstract
The study of the development of the vertebrate retina can be addressed from several perspectives: from purely qualitative to a more quantitative approach that takes into account its spatiotemporal features, its three dimensional structure and also the regulation and properties at the systems level. Here we review the ongoing transition towards a full four-dimensional characterization of the developing vertebrate retina, focusing on the challenges at the experimental, image acquisition, image processing and quantification. Using the developing zebrafish retina, we illustrate how quantitative data extracted from these type of highly dense three-dimensional tissues depends strongly on the image quality, image processing and algorithms used to segment and quantify. Therefore, we propose that the scientific community that focuses on developmental systems could strongly benefit from a more detailed disclosure of the tools and pipelines used to process and analyze images from biological samples.
Keywords
Retinogenesis; Quantiative Biology; Imaging
Subject
Biology and Life Sciences, Anatomy and Physiology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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