Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Loop-Mediated Isothermal Amplification in Core-Shell Beads Assay for the Detection of Tyrosine Kinase AXL Over Expression

Version 1 : Received: 7 July 2021 / Approved: 8 July 2021 / Online: 8 July 2021 (10:57:31 CEST)

How to cite: Sreejith, K.R.; Umer, M.; Singha, P.; Nguyen, N.; Kasetsirikul, S.; Ooi, C.H.; Shiddiky, M.; Nguyen, N. Loop-Mediated Isothermal Amplification in Core-Shell Beads Assay for the Detection of Tyrosine Kinase AXL Over Expression. Preprints 2021, 2021070195 (doi: 10.20944/preprints202107.0195.v1). Sreejith, K.R.; Umer, M.; Singha, P.; Nguyen, N.; Kasetsirikul, S.; Ooi, C.H.; Shiddiky, M.; Nguyen, N. Loop-Mediated Isothermal Amplification in Core-Shell Beads Assay for the Detection of Tyrosine Kinase AXL Over Expression. Preprints 2021, 2021070195 (doi: 10.20944/preprints202107.0195.v1).

Abstract

The upregulated expression of thyrosine kinase AXL has been reported in several hematologic and solid human tumors including gastric, breast, colorectal, prostate, and ovarian cancers. Thus, AXL can potentially serve as a diagnostic and prognostic biomarker for various cancers. This paper reports the first-ever use of loop-mediated isothermal amplification (LAMP) of the AXL gene as a diagnostic method for ovarian cancer. We demonstrated simple instrumentation toward a point-of-care device to perform LAMP. This paper also reports the first-ever use of core-shell beads as a microreactor to perform LAMP as an attempt to promote environmentally friendly laboratory practices.

Subject Areas

Loop-mediated isothermal amplification; AXL over expression; core-shell beads assay

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