Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Loop-Mediated Isothermal Amplification in Core-Shell Beads Assay for the Detection of Tyrosine Kinase AXL Over Expression

Version 1 : Received: 7 July 2021 / Approved: 8 July 2021 / Online: 8 July 2021 (10:57:31 CEST)

A peer-reviewed article of this Preprint also exists.

Sreejith, K.R.; Umer, M.; Singha, P.; Nguyen, N.-K.; Kasetsirikul, S.; Ooi, C.H.; Shiddiky, M.J.A.; Nguyen, N.-T. Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression. Micromachines 2021, 12, 905. Sreejith, K.R.; Umer, M.; Singha, P.; Nguyen, N.-K.; Kasetsirikul, S.; Ooi, C.H.; Shiddiky, M.J.A.; Nguyen, N.-T. Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression. Micromachines 2021, 12, 905.

Abstract

The upregulated expression of thyrosine kinase AXL has been reported in several hematologic and solid human tumors including gastric, breast, colorectal, prostate, and ovarian cancers. Thus, AXL can potentially serve as a diagnostic and prognostic biomarker for various cancers. This paper reports the first-ever use of loop-mediated isothermal amplification (LAMP) of the AXL gene as a diagnostic method for ovarian cancer. We demonstrated simple instrumentation toward a point-of-care device to perform LAMP. This paper also reports the first-ever use of core-shell beads as a microreactor to perform LAMP as an attempt to promote environmentally friendly laboratory practices.

Keywords

Loop-mediated isothermal amplification; AXL over expression; core-shell beads assay

Subject

Chemistry and Materials Science, Analytical Chemistry

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.