Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

On the Applicability of Electrophoresis for Protein Quantification

Version 1 : Received: 5 July 2021 / Approved: 6 July 2021 / Online: 6 July 2021 (14:54:12 CEST)

How to cite: Dome, K.; Akimenko, Z.; Bychkov, A.; Kalambet, Y.; Lomovsky, O. On the Applicability of Electrophoresis for Protein Quantification. Preprints 2021, 2021070159 (doi: 10.20944/preprints202107.0159.v1). Dome, K.; Akimenko, Z.; Bychkov, A.; Kalambet, Y.; Lomovsky, O. On the Applicability of Electrophoresis for Protein Quantification. Preprints 2021, 2021070159 (doi: 10.20944/preprints202107.0159.v1).

Abstract

Polyacrylamide gel electrophoresis (PAGE) is widely used for studying proteins and protein-containing objects. However, it is employed most frequently as a qualitative method rather than a quantitative one. In this paper, we show the feasibility of routine digital image acquisition and mathematical processing of electrophoregrams for protein quantification. Both the well-studied model protein molecules (bovine serum albumin) and more complex real-world protein-based products (casein-containing isolate for sports nutrition), which were subjected to mechanical activation in a planetary ball mill to obtain samples characterized by different protein denaturation degrees, were used as study objects. Protein quantification in the mechanically activated samples was carried out. The degree of destruction of individual protein was shown to be higher compared to that of protein-containing mixture after mechanical treatment for an identical amount of time. The methodological approach used in this study can serve as guidance for other researchers who would like to use electrophoresis for protein quantification both in individual form and in protein mixtures. The findings prove that photographic imaging of gels followed by mathematical data processing can be applied for analyzing the electrophoretic data.

Subject Areas

electrophoresis; protein; mechanical treatment; quantification

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.