Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Metabolomics Response to Drought Stress in Morus alba L. variety Yu-711

Michael Ackah
ORCID logo ,
Yisu Shi
,
Mengmeng Wu
,
Lei Wang
,
Guo Peng
,
Guo Liangliang
,
Jin Xin
,
Li Shaocong
,
Zhang Qiaonan
,
Changyu Qiu
,
Qiang Lin
,
Weiguo Zhao
*
Version 1 : Received: 1 July 2021 / Approved: 5 July 2021 / Online: 5 July 2021 (15:47:17 CEST)
Version 2 : Received: 6 July 2021 / Approved: 6 July 2021 / Online: 6 July 2021 (17:23:32 CEST)

A peer-reviewed article of this Preprint also exists.

Ackah, M.; Shi, Y.; Wu, M.; Wang, L.; Guo, P.; Guo, L.; Jin, X.; Li, S.; Zhang, Q.; Qiu, C.; Lin, Q.; Zhao, W. Metabolomics Response to Drought Stress in Morus alba L. Variety Yu-711. Plants 2021, 10, 1636. Ackah, M.; Shi, Y.; Wu, M.; Wang, L.; Guo, P.; Guo, L.; Jin, X.; Li, S.; Zhang, Q.; Qiu, C.; Lin, Q.; Zhao, W. Metabolomics Response to Drought Stress in Morus alba L. Variety Yu-711. Plants 2021, 10, 1636.

Abstract

Mulberry is an economically significant crop for the sericulture industry worldwide. Stresses such as drought exposure have a significant influence on plant survival. Metabolome directly reflects plant physiological status; thus, a way to assess this impact is to perform a global metabolomic analysis. This study investigated the effect of drought stress on mulberry Yu-711 metabolic balance using a liquid chromatography-mass spectrometry (LC-MS) based on an untargeted metabolomic approach. For this objective, Yu-711 leaves were subjected to two weeks of drought stress treatment and control without drought stress. Multivariate and univariate statistical analyses highlighted numerous differentially-accumulated metabolic elements as a function of time and treatment. Drought stress led to a more differentiated metabolites response than the control. We found that the levels of total lipids and galactolipids, and phospholipids (PC, PA, PE) were significantly altered, producing 48% of the total differentially expressed metabolites. Fatty acyls were the most abundant lipids expressed and decreased considerably by 73.6%. Prenol lipids class of lipids increased in drought leaves. Other classes of metabolites, including polyphenols( flavonoids and cinnamic acid), organic acid (amino acids), carbohydrates, benzenoids, and organoheterocyclic, all had a dynamic trend in response to the drought stress. However, their levels under drought stress generally decreased significantly compared to the control. These results provide an overview of the metabolic profile of the mulberry plant through differentially-accumulated compounds and provide a better understanding of global plant metabolic changes in defense mechanisms.

Keywords

untargeted approach; metabolites; drought stress; mulberry, LC-MS

Subject

Biology and Life Sciences, Anatomy and Physiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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