Working Paper Article Version 1 This version is not peer-reviewed

Exogenous Gut Microbiota Influence Fiber Size and Energy Metabolism Characteristics in Skeletal Muscle of Mice

Version 1 : Received: 14 June 2021 / Approved: 17 June 2021 / Online: 17 June 2021 (14:22:45 CEST)

How to cite: Li, M.; Zhang, X.; Zhu, X.; Hou, Q.; Cai, L. Exogenous Gut Microbiota Influence Fiber Size and Energy Metabolism Characteristics in Skeletal Muscle of Mice. Preprints 2021, 2021060461 Li, M.; Zhang, X.; Zhu, X.; Hou, Q.; Cai, L. Exogenous Gut Microbiota Influence Fiber Size and Energy Metabolism Characteristics in Skeletal Muscle of Mice. Preprints 2021, 2021060461

Abstract

The objective of this study was to investigate the effects of exogenous gut microbiota influence fiber size and energy metabolism characteristics in skeletal muscle of mice. Firstly, we compared the differences in muscle fiber diameter, fiber density, and gut microbiota composition between Erhualian pigs (EH) and Landrace×Yorkshire crossed pigs (LY). It is found that EH exhibited thinner fiber diameters and higher fiber densities (P < 0.05) of skeletal muscle compared with that of LY. Moreover, 8 gut microbial phyla and 35 genera were significantly different (P < 0.05) between EH and LY. Correlation analysis revealed all those significantly different microbial genera were significantly correlated with skeletal muscle fiber diameters and densities. Next, 24 newborn mice were divided into 2 groups (CG and AG) treated with sterilized saline and fecal microbiota suspensions from EH, respectively, for 90 days. It is revealed that the relative abundance of Parabacteroides, Sutterlla, and Dehalobacterium were significantly increased in the fecal of AG compared with that of CG. These microbiota contributing to improved functions related to carbohydrate, lipid, and vitamin metabolism. The weight, the fiber densities, and gene expression related to the growth, differentiation, fiber types, mitochondrial function, and carbohydrate metabolism were significantly increased, while the fiber diameters were significantly decreased in the skeletal muscle of AG compared with that of CG. These results suggested that exogenous gut microbiota influence the weight, fiber size and energy metabolism in skeletal muscle of mice via reshaped their gut microbiota composition.

Subject Areas

pigs; mice, gut microbiota; skeletal muscle; fiber size, energy metabolism

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