Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Staphylococcus aureus Protection-Related Type 3 Cell-Mediated Immune Response Elicited by Recombinant Proteins and GM-CSF DNA Vaccine

Kamila R. Santos
* ,
Fernando N. Souza
,
Eduardo R. Sanchez
ORCID logo ,
Camila F. Batista
,
Luiza C. Reis
,
Wesley F. Fotoran
,
Marcos B. Heinemann
ORCID logo ,
Hiro Goto
,
Magnus Gidlund
ORCID logo ,
Adriano F. Cunha
ORCID logo ,
Angélica Rosa Faria
,
Hélida M. Andrade
,
Andrey P. Lage
,
Mônica M. O. P. Cerqueira
,
Alice M.M.P. Della Libera
ORCID logo
Version 1 : Received: 3 June 2021 / Approved: 7 June 2021 / Online: 7 June 2021 (07:54:02 CEST)

A peer-reviewed article of this Preprint also exists.

Santos, K.R.; Souza, F.N.; Ramos-Sanchez, E.M.; Batista, C.F.; Reis, L.C.; Fotoran, W.F.; Heinemann, M.B.; Goto, H.; Gidlund, M.; Cunha, A.F.; Faria, A.R.; Andrade, H.M.; Lage, A.P.; Cerqueira, M.M.O.P.; Della Libera, A.M.M.P. Staphylococcus aureus Protection-Related Type 3 Cell-Mediated Immune Response Elicited by Recombinant Proteins and GM-CSF DNA Vaccine. Vaccines 2021, 9, 899. Santos, K.R.; Souza, F.N.; Ramos-Sanchez, E.M.; Batista, C.F.; Reis, L.C.; Fotoran, W.F.; Heinemann, M.B.; Goto, H.; Gidlund, M.; Cunha, A.F.; Faria, A.R.; Andrade, H.M.; Lage, A.P.; Cerqueira, M.M.O.P.; Della Libera, A.M.M.P. Staphylococcus aureus Protection-Related Type 3 Cell-Mediated Immune Response Elicited by Recombinant Proteins and GM-CSF DNA Vaccine. Vaccines 2021, 9, 899.

Abstract

Staphylococcus aureus mastitis remains a major challenge for dairy farming. Here, 24 mice were immunized and divided into four groups: G1: control; G2: Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF) DNA vaccine; G3: F0F1 ATP synthase subunit α (SAS), succinyl-diaminopimelate (SDD), and cysteinyl-tRNA synthetase (CTS) recombinant proteins; and G4: SAS+SDD+CTS plus GM-CSF DNA vaccine. The lymphocyte subpopulations and the intracellular interleukin-17A (IL-17A) and interferon-γ production in the draining lymph node cells were immunophenotyped by flow cytometry. The immunophenotyping and lymphocyte proliferation was determined in spleen cells cultured with and without S. aureus stimulus. Immunization with S. aureus recombinant proteins generated memory cells in draining lymph nodes. Immunization with the three recombinant proteins plus GM-CSF DNA led to an increase in the percentage of IL-17A+ cells among overall CD44+ (memory), T CD4+, CD4+ T CD44+ CD27-, γδ TCR, γδ TCR+ CD44+ CD27+ and TCRVγ4+ cells. Vaccination with S. aureus recombinant proteins associated with GM-CSF DNA vaccine downregulates TH2 immunity. Immunization with the three recombinant proteins plus the GM-CSF DNA led to a proliferation of overall memory T, CD4+ and CD4+ TEM cells upon S. aureus stimulus. This approach fostered type 3 immunity, suggesting the development of a protective immune response against S. aureus.

Keywords

vaccine; Staphylococcus aureus; T cell response; mastitis; bovine

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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